REBASE-The Restriction Enzyme Database

描述（由申请人提供）： 该项目涉及一个关于限制性内切核酸酶及其相关甲基转移酶的连贯和全面的信息数据库的维护和分发。该数据库的主要组成部分是关于限制性内切核酸酶的甲基敏感性的信息和对测序的微生物基因组中存在的限制性内切酶的全面分析。表演场地是位于马萨诸塞州贝弗利的新英格兰生物实验室。 REBASE自1975年以来一直存在，目前以关系格式在 SYBASE。数据库中的信息来自已发表的文献，以及研究者在发表前或未发表时直接提交的信息。大约10%的描述实验数据的条目没有通过REBASE发表。中的信息 REBASE通过科学文献的正式出版物和各种电子格式在全世界传播。我们每月发布更新，并通过自动电子邮件列表将选定的文件分发到400多个站点。此外，还可通过匿名FTP或通过万维网获取信息。 在每月维护和更新的文件中，有一些专门的文件， 更广泛使用的计算机程序，如GCG，McVector，DNA Strider，LaserGene等。这些文件随时可用，包括有关限制性内切酶全系列的最新信息。 特性及其商业可用性。通过REBASE获得的信息是所有人的关键。 研究人员计划进行切割和/或重新连接DNA的实验。它是生物技术实践的独特资源，其内容直接或间接地被研究卫生保健界的许多部门使用。

DESCRIPTION (provided by applicant): This project concerns the maintenance and distribution of a cohesive and comprehensive database of information about restriction endonucleases and their associated methyltransferases. Key components of the database are information about the methyl sensitivity of restriction endonucleases and a comprehensive analysis of the restriction enzymes present in the sequenced microbial genomes. The performance site is New England Biolabs, located in Beverly, MA. REBASE has been in existence since 1975 and is currently implemented in a relational format in SYBASE. Information enters the database both from the published literature and by direct submission from the investigators either prior to publication or instead of publication. Approximately 10% of the entries describing experimental data are unpublished other than through REBASE. The information in REBASE is disseminated throughout the world both by formal publication in the scientific literature and in a variety of electronic formats. We post monthly updates and selected files are distributed through an automatic email list to more than 400 sites. In addition, the information is available by anonymous FTP or through the worldwide web. Among the files maintained and updated monthly, are specialized ones that are used by many of the more widely used computer programs such as GCG, McVector, DNA Strider, LaserGene, etc. These files are ready to use and include up-to-date information about the full range of restriction endonuclease specificities and their commercial availabilities. Information available through REBASE is key to all investigators planning experiments to cleave and/or religate DNA. It is a unique resource for the practice of biotechnology and its contents are used directly or indirectly by many sections of the research health care community.