Transgenic Mouse Facility

国家癌症研究所的转基因小鼠模型（TMM）实验室弗雷德里克通过向受精卵进行原核微注射来生产转基因小鼠，为需要研究体内基因功能的研究人员提供帮助。虽然这项服务是根据每个客户的需求定制的，但这项工作的核心包括纯化待微注射的DNA片段，并通过Southern blot、PCR或槽点blot对所得小鼠进行分析。用于转基因小鼠研究的重组DNA结构的设计咨询是可用的。在RNA或蛋白质水平上对转基因表达模式的表征也是可用的。转基因小鼠通常在FVB/N和C57BL/6背景下产生。

The Transgenic Mouse Model (TMM) Laboratory at the National Cancer Institute ¿ Frederick provides assistance to investigators with a need to study in vivo gene function, by producing transgenic mice by pronuclear microinjection of fertilized mouse eggs. Although this service is customized to the needs of each client, the core of this effort includes purification of the DNA fragment to be microinjected and analysis of the resulting mice by Southern blot, PCR, or slot/dot blot. Consultations for the design of recombinant DNA constructs for the use in transgenic mouse studies are available. Characterization of the pattern of transgene expression at the RNA or protein level is also available. Transgenic mice are routinely produced in FVB/N and C57BL/6 backgrounds.