DCTD Natural Products Extraction

Thomas McCloud领导下的这个实验室的主要职能是从天然产品来源(海洋无脊椎动物、植物和微生物)中制备提取物，目的是生产用于墙内和墙外实验室和地点的癌症相关研究的材料。随后，这些材料被镀在96和384孔板中，并生产溶解材料，包括提取物和合成化合物，用于随后的60细胞系癌细胞分析和其他DTP感兴趣的分子靶标分析，以支持抗癌药物的开发。此外，实验室对来自各种抗癌检测的检测结果执行复制功能，从而能够迅速做出关于特定检测结果的继续工作的决定。作为去复制工作的结果，已经积累了大量的分级板，因此如果相同或相似的提取物在与癌症相关的不同检测中命中，则可以相对容易地获得分馏前的样本。 作为癌症药物发现和开发的一部分，该实验室还用于对潜在抗癌药物进行快速质量控制和鉴定工作，这些药物来自开发治疗计划的各种感兴趣来源。也有能力从各种来源分离和提纯感兴趣的小分子，并在必要时通过半合成来执行一些结构活性工作。除了与粗提物相关的体内检测外，随着这些结果的公布，实验室将对HITS进行初步的化学评估。 随着化学生物学联盟倡议的加强，该实验室将成为该过程中一些初始步骤的焦点；即提取物制备和平板生产、初始去复制，以及在某些情况下从各种分析中鉴定先导化合物。

The major functions of this laboratory under Thomas McCloud are related to the preparation of extracts from natural product sources (marine invertebrates, plants and microbes) with the aim of producing materials for use in cancer related studies both in intramural and extramural laboratories and locations., The materials are subsequently ¿plated¿ in 96 and 384 well plates, and the production of solubilized materials, both extracts and synthetic compounds for subsequent 60 cell line cancer cell assays and other, usually molecular target assays of interest to the DTP in support of cancer drug development. In addition, the laboratory performs a ¿dereplication¿ function for hits from various anti-cancer assays, thus enabling a decision to be rapidly made concerning continuation of work on a particular ¿hit¿. As a result of the dereplication effort, significant numbers of ¿fraction plates¿ have been amassed thus permitting relatively easy access to prefractionated samples if the same or similar extracts ¿hit¿ in a different assay related to cancer. The laboratory also is utilized for rapid Quality Control and identification work on potential anticancer agents from a variety of sources of interest to the Developmental Therapeutics Program as part of cancer drug discovery and development. There is also the capability to isolate and purify small molecules of interest from a variety of sources and to perform some structure activity work via semisynthesis when/where necessary. In addition with the in vivo assays related to crude extracts, as these results come in, the laboratory will be performing initial chemical assessments on the hits. As the Chemical Biology Consortium initiative ramps up, this laboratory will become the focal point for a number of initial steps in that process; viz extract preparation and plate production, initial dereplication and in certain cases identification of lead compounds from a variety of assays.