BIOMOLECULE ANALYSIS CORE FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们建议继续支持我们的多用户研究设施，专门从事生物分子分析，生物分子分析核心设施（BACF）。BACF拥有先进的设备，如质谱仪，nanoLC系统，表面等离子体共振（BIACORE），HPLC系统，荧光计和光度计，超浓缩仪，制备离心机和其他几个共享仪器。许多其他资源可用于记录和分析使用这些仪器收集的实验数据。该核心的长期目标是为尽可能广泛的用户群体提供维护良好的尖端生物分子分析设备。该设施现在位于新的生物科学大楼，占地1,650平方英尺。BACF目前由7个UTEP部门和位于埃尔帕索的休斯顿公共卫生学院使用，代表50个研究小组，40名教职员工，18名研究助理（工作人员），16名博士后，48名博士生，32名MS学生和128名本科生。目前，该核心平均每月访问1，000次（约1，000次）。每年12，000个），由249个个人用户。Core配备了一名全职技术员（协调员），负责工厂技术员和工程师的日常维护计划预防性维护，用户指导和帮助，以及帐户和库存维护。将向监督这些活动的核心主任提供部分薪金。为了实现该设施的自我维持维护，我们计划向UTEP/BBRC和外部用户提供新的核心服务，例如通过LC-MS/MS进行肽测序/蛋白质组学分析。为此，我们在这里申请资金，聘请一名全职研究助理或技术员，在博士水平，以监督质谱和蛋白质组学分析活动。为了成功实现我们的目标，提出了三个具体目标：具体目标#1：继续为BBRC研究人员和外部用户提供维护良好的最先进设备。具体目标2：继续提供核心设备使用方面的技术指导支持。具体目标3：提供旨在自我维持核心的服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose continuing support for our multi-user research facility specializing in the analysis of biomolecules, the Biomolecule Analysis Core Facility (BACF). The BACF houses cutting-edge equipment, such as mass spectrometers, nanoLC systems, surface plasmon resonance (BIACORE), HPLC systems, spectrophotometers, fluorometer and luminometer, ultracentrifuges, preparative centrifuges, and several other shared instruments. Numerous other resources are available for documentation and analysis of experimental data collected using these instruments. The long-term objective of this Core is to provide the broadest possible group of users with access to well-maintained, cutting-edge equipment for biomolecule analysis. The facility is now housed in the new Bioscience Building, in an area of 1,650 sq feet. The BACF is currently used by 7 UTEP departments and the School of Public Health Houston at El Paso, representing 50 research groups, 40 faculty members, 18 research assistants (staff), 16 post-docs, 48 PhD students, 32 MS students, and 128 undergraduate students. The Core is currently accessed in average 1,000 times per month (approx. 12,000 per year), by 249 individual users. The Core is staffed by a full-time technician (coordinator), who is responsible for routine maintenance scheduling preventive maintenance by factory technicians and engineers, user orientation and assistance, as well as account and inventory maintenance. Partial salary will be provided to the core director for overseeing these activities. Aiming at a self-sustaining maintenance of the facility, we plan to offer new core services, such as peptide sequencing/proteomic analysis by LC-MS/MS, to UTEP/BBRC and external users. For this, here we request funds for hiring one full-time research associate or technician, at PhD level, to oversee the mass spectrometry and proteomic analysis activities. To successfully achieve our objectives, three specific aims are proposed: Specific Aim #1: Continue to provide BBRC researchers and external users access to well-maintained, state-of-the-art equipment. Specific Aim #2: Continue to provide technical instructional support in the use of Core equipment. Specific Aim # 3: To provide services aiming at a self-sustaining maintenance of the Core.