Mouse Core

Brandeis在无脊椎动物模式生物的神经遗传学方面有着悠久而丰富的历史，但只在最后一段时间 两年来，我们是否开始充分利用现有的转基因小鼠品系和 自己开发新的。以前在纳尔逊、特里加诺、比伦和利斯曼发现的哺乳动物 实验室主要是在老鼠身上进行的。由于建立了鼠标/病毒核心设施 在老鼠身上的强大的新的基因敲除和转基因方法现在都是研究计划的一部分。 这些最先进的方法产生了新的合作 布兰代斯教职员工。例如，Birren和Sengupta正在研究与 转录因子Arx在蠕虫和小鼠中，他们已经开始研究有条件地敲除Arx在 老鼠。纳尔逊实验室已经产生了新的转基因株系，可以标记和操纵 特定的前脑细胞类型。第一条特征线标记原代第4层星形锥体神经元 感觉皮质。它将在改变的分子基础上加强与特瑞吉亚诺的合作项目 视皮层第四层的突触可塑性形式。新的小鼠是用慢病毒制成的 转基因和反映新开发的设施创建和收集慢病毒载体的能力 脑转基因和脑转基因。为了优化慢病毒对脆弱组织的输送，我们 建议购买Burliegh Piezo冲击钻机系统。Brandeis教职员工的新成员将 也要利用这个设施。卡茨正在将他的大鼠多电极记录技术应用于小鼠，并将 与Birren、Nelson和Turrigiano合作进行多个项目，对转基因小鼠进行体内分析 模特们。帕拉迪斯将于2008年1月加入教职；她将使用该设施制造基因敲除小鼠 对分析谷氨酸和GABA能突触的关键调节分子很重要 哺乳动物中枢神经系统的发育。其中包括4类信号素，她在大范围内确定了这一类 RNAi屏幕1。 对该设施的持续支持将使这些调查人员能够获得足够的初步结果 寻求额外的NINDS资金。

Brandeis has a long and rich history of neurogenetics in invertebrate model organisms, but only in the last two years, have we begun to begin to take full advantage of existing transgenic mouse strains and develop new ones ourselves. Prior mammalian work in the Nelson, Turrigiano, Birren and Lisman laboratories has been primarily conducted in rats. Thanks to establishment of the Mouse/Virus Core facility powerful new knockout and transgenic approaches in mice are now part of the research program in each of these laboratories, and these state-of-the-art approaches have engendered new collaborations between Brandeis faculty. For example, Birren and Sengupta are studying signaling associated with the transcription factor Arx in worms and mice, and they have begun work on a conditional knockout of Arx in mice. The Nelson lab has generated new transgenic lines which allow labeling and manipulation of specific forebrain cell types. The first characterized line labels layer 4 star-pyramid neurons in primary sensory cortices. It will enhance a collaborative project with Turrigiano on the molecular basis of changing forms of synaptic plasticity in layer 4 of visual cortex. The new mice have been made using lentiviral transgenesis and reflect the facilities newly developed capability to creat and harvest lentiviral vectors for brain transfection and transgenesis. In order to optimize delivery of lentivirus to delicate tissues we proposed to purchase a Burliegh Piezo Impact Drill system. Newer additions to the Brandeis faculty will also make use of the facility. Katz is adapting his rat multielectrode recording techniques to mice and will collaborate in multiple projects with Birren, Nelson and Turrigiano on in vivo analyses of transgenic mouse models. Paradis will join the faculty in January 2008; she will use the facility to make knockout mice important for analyzing molecules that are critical regulators of glutamatergic and GABAergic synapse development in the mammalian CNS. These include class 4 Semaphorins, which she identified in a largescale RNAi screen 1. Continued support of the facility would permit these investigators to obtain sufficient preliminary results to seek additional NINDS funding.