MACROMOLECULAR CRYSTALLIZATION, SAMPLE PREPARATION AND SYNCHROTRON DATA COLLECTI

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本次研讨会的目的是为生物观众谁要么目前利用SSRL大分子晶体学光束线，以获得感兴趣的目标的结构信息，或有计划启动一些晶体学工作使用SSRL光束线。讲习班的重点是为那些在近期和中期内将受益于结构信息的项目提供实用的培训。讲座内容包括晶体学基础知识，强调优质晶体的重要性，概述豪普特曼-伍德沃德医学研究所（HWI）向公众提供的高通量筛选服务及其在可溶性蛋白和最近膜蛋白中的应用，优化实验室初始结晶条件，沿着为同步加速器准备晶体。随后进行了实际操作，介绍了SSRL远程数据收集设施（包括Blu-Ice和Web-Ice）的使用情况，并利用现有工具对晶体进行低温冷却、储存和运输，以收集尽可能好的X射线数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This workshop was intended for a biological audience who either currently makes use of SSRL macromolecular crystallography beam lines to obtain structural information on targets of interest, or had plans to initiate some crystallographic work using SSRL beam lines. The workshop was focused at a practical level for those with projects that would benefit from structural information in the near to intermediate term. Lectures covered the basics of crystallography, emphasizing the importance of good quality crystals, an overview of the high-throughput screening service available to the general community at the Hauptman-Woodward Medical Research Institute (HWI) and its application to both soluble and very recently membrane proteins, optimization of initial crystallization conditions in the laboratory, along with getting crystals ready for the synchrotron. Hands-on practical sessions followed, describing the use of remote data collection facilities at SSRL (including Blu-Ice and Web-Ice) and making use of the tools available to cryo-cool, store and transport the crystals to SSRL in order to collect the best x-ray data possible.