DEVELOPMENT AND COMMISSIONING OF MICROBEAM CAPABILITIES ON BL12-2

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 用于研究微晶的微束模式正在开发和实施中。 内部开发并安装了精密微衍射仪。 该系统基于单旋转轴高精度空气轴承，并采用3度运动，用于样品对准，具有1微米的再现性和1.4微米的混淆球（SOC）。 该系统已热稳定与保护铜热屏蔽纳入身体的衍射仪。 实验台已进行热补偿，并且将箱温度控制在+/- 0.5度。 一个在线摄像机被纳入光束线，用于观察微晶体沿着光束轴，这减少了由于视差的错位。 该相机可以解决1微米的特点，并纳入3度的运动，便于对齐和变焦功能（8倍）。 一个弹出屏幕已被包括在内，它提供了背光的小样本和荧光屏，用于识别光束相对于样本的位置。 该相机已被纳入蓝冰控制系统，并与远程访问兼容，最近测试了一个原型自动准直系统。 该系统允许自动选择小光束尺寸（小于55 um x 20 um的原始焦点）。 微型准直器固定在同轴摄像机透镜前面的小塑料条上。 然而，这些条带不会使在线图像失真到任何显著程度。 一个新的系统正在制造，将允许选择4个光束尺寸范围从5-20微米。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Microbeam mode for studying microcrystals is being developed and implemented. A precision microdiffractometer was developed in-house and installed. The system is based on a single rotation axis high-precision air bearing and incorporates 3 degrees of motion for sample alignment with 1 micron reproducibility and 1.4 micron sphere of confusion (SOC). The system has been thermally stabilized with a protective copper heat shield incorporated in the body of the diffractometer. The experimental table has been thermally compensated and the hutch temperature is controlled to +/- 0.5 degrees. An in-line camera was incorporated into the beam line for viewing microcrystals along the beam axis, which reduces misalignments due to parallax. The camera can resolve 1 micron features and incorporates 3 degrees of motion for easy alignment and a zoom feature (8x). A pop-in screen has been included which provides backlight for the small samples and a fluorescent screen for identifying the beam position relative to the sample. The camera has been incorporated into the Blu-Ice control system and is compatible with remote access, etc. A prototype automated collimation system was recently tested. The system allows the automatic selection of small beam sizes (smaller than the native focus of 55 um x 20 um). The tiny collimators are held in place on small plastic strips just in front of the in-line camera lens. However these strips do not distort the in-line image to any significant extent. A new system is being fabricated that will allow the selection of 4 beam sizes ranging from 5-20 microns.