A novel fragmentation technique for tandem mass spectrometry

描述（由申请人提供）：本提案的主要目标是开发一种基于肽离子与自由基束相互作用的新型碎片技术。我们计划证明，这种技术将产生不同类型的碎片离子相比，在碰撞诱导解离。它还将克服与碰撞诱导解离相关的问题，例如，在许多重要的翻译后修饰中涉及的不稳定基团的优先断裂和易丢失。将这两种碎片化技术结合在一个高性能的QqTOF质谱仪中，将大大提高这些仪器在高通量蛋白质组学应用中的价值。

DESCRIPTION (provided by applicant): The primary goal of this proposal is to develop a novel fragmentation technique based on interaction of peptide ions with radical beam. We plan to demonstrate that this technique will produce different type of fragment ions compared to those obtained in collision-induced dissociation. It will also overcome problems associated with collision-induced dissociation, e.g. preferential fragmentation and facile losses of labile groups involved in many important posttranslational modifications. Combining both fragmentation techniques in one high performance QqTOF mass spectrometer will substantially increase the value of these instruments for high throughput proteomics applications. PUBLIC HEALTH RELEVANCE: Proteomics studies biological processes by the systematic analysis of the proteins expressed in a cell or tissue. It plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new high throughput technology for increasing the efficiency of protein identification and structure elucidation using tandem mass spectrometry.