EVALUATION OF SAMPLING FREQUENCIES OF BETA ENDORPHINS IN PCOS WOMEN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 多囊卵巢综合征(PCOS)导致少排卵，常伴有雄激素升高(多毛症和/或痤疮)、胰岛素抵抗和黄体生成素(LH)升高。多囊卵巢综合征通常用药物(口服避孕药、二甲双胍、生育药物)治疗。在我们追求针灸作为一种非药物治疗PCOS的生物学机制的过程中，对β-内啡肽(Endo)的作用知之甚少。黄体生成素由大脑以脉动的方式释放，但在多囊卵巢综合征患者中整个月都以高水平分泌。在正常排卵的女性中，Endo也是搏动性的，抑制促黄体生成素，而在PCOS患者中，Endo通常比正常骑自行车的女性更高。我们的针灸随机临床试验的初步数据显示，多囊卵巢综合征患者排卵时，内分泌水平恢复正常，黄体生成素水平降低。为了提高我们设计更大规模研究的能力，我们将收集未经治疗的PCOS妇女Endo分泌脉冲特征的初步数据。具体地说，这项研究将在标准化的8小时内收集4名未经治疗的多囊卵巢综合征妇女的连续血样(Q10血样)。血清测定采用超灵敏化学发光法(LH1)和柱层析/酶联免疫吸附试验(Endo)。我们的目标是a)评估不同的采样范例，希望在未来的研究中减少内分泌血液采样的频率；(B)评估内分泌和促黄体生成素分泌脉冲特征之间的关系；c)描述未经治疗的多囊卵巢综合征妇女的内分泌脉冲特征。这项建议对我们未来研究的意义在于财务(Endo分析的费用较高，每样本折扣研究价格为55美元，而卵巢激素每样本折扣研究价格为3美元)和实用性(Endo所需的血量为4毫升，而黄体生成素为1毫升)。这一知识对于我们关于等待重新提交的多囊卵巢综合征的针灸生物学机制的R01拨款的预算和协议至关重要(AT004700)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Polycystic Ovary Syndrome (PCOS) causes oligo-ovulation, and is often accompanied by elevated androgens (hirsutism and/or acne), insulin resistance, and elevated luteinizing hormone (LH). PCOS is usually treated with pharmaceuticals (oral contraceptives, metformin, fertility drugs). In our pursuit of the biological mechanism underlying acupuncture as a non-drug treatment for PCOS, there is a gap of knowledge on the role of beta-endorphins ( Endo). LH is released in a pulsatile fashion by the brain, but is secreted at high levels throughout the month in PCOS. Endo is also pulsatile, acts to suppress LH in normo-ovulatory women, and Endo is generally higher in PCOS than normally cycling women. Preliminary data from our acupuncture randomized clinical trial indicates that Endo levels normalize and LH levels are reduced in PCOS women when they become ovulatory. In order to improve our ability to design a larger study, we will gather preliminary data on the secretory pulse characteristics of Endo in untreated PCOS women. Specifically, this study will collect continuous blood samples over a standardized 8-hour time period (q10 blood sampling) in 4 untreated PCOS women. Serum will be assayed by ultrasensitive chemiluminescence (LH,) and column chromatography/ELISA ( Endo) assays. Our aims are to a) assess varying sampling paradigms in hopes of reducing the frequency of blood sampling for Endo in future studies; (b) assess the relationship between Endo and LH secretory pulse characteristics; c) describe the Endo secretory pulse characteristics in untreated PCOS women. The significance of this proposal for our future research is financial (high expense of Endo assays at $55/sample discounted research price compared with ovarian hormones at $3/sample discounted research price) and practical (volume of blood needed for Endo is 4 mL compared with 1 mL for LH). This knowledge is critical to the budget and protocol for our R01 grant on the biological mechanism of acupuncture for PCOS awaiting resubmission (AT004700).