ESTABLISHMENT OF A SPF RHESUS MACAQUE COLONY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 描述(由申请人提供)：该项目的目标是通过维持一个生产B型疱疹病毒、SIV、SRV和STLV-1的印度源恒河猴的生产群体，提高该国生产无家系特定病原体(SPF)猕猴用于艾滋病相关研究的能力，并通过提供与MHC基因相关的动物来满足近期和长期的特定研究需求。具体目标如下：1.通过一个详细的评估和剔除阳性动物的计划，为NIH资助的研究人员提供以下动物数量：06年60只，07年60只，08年70只，09年70只，10年70只。种群将扩大到稳定的种群约430只，其中雌性繁殖者约140只，雄性繁殖者约20只，婴幼儿和青少年约275只，使每年继续收获约70只动物用于研究；2.通过向调查人员提供与以下MAMU等位基因相关的动物：MAMU-A*01、-A*02、-A*11、-B*01、-6*03、-B*04和-B*17，最大化动物用于研究目的的价值；3.通过a)建立、管理和利用用于群体遗传管理的系谱数据库，最大化群体生产的长期效率。通过NIH/NCRR的其他资金在头5年开发的20个高度信息量的微卫星多态，以及建立这个数据库的人口统计记录，将用于建立和验证系谱信息，管理繁殖以避免近亲繁殖和保持遗传变异，同时重点关注通过利用群体中的死亡率、发病率和生育力数据最大化生产和收获计划的主要目标。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DESCRIPTION (provided by applicant): The goals of this project are to increase the nation's capacity to produce pedigreed specific pathogen free (SPF) Macaca mulatta for AIDS-related research by maintaining a production colony of Indian-origin rhesus monkeys that are SPF for herpes B virus, SIV, SRV, and STLV-1, and to fulfill specific research needs in the immediate and long-term future by providing animals characterized relative to MHC genotype. The specific aims are as follows: 1. Through a detailed program of assessment and removal of positive animals, to provide the following numbers of animals to NIH-funded investigators: 60 in Year 06, 60 in Year 07, 70 in Year 08, 70 in Year 09 and 70 in Year 10. The colony will expand to a steady-state population of around 430 animals, with approximately 140 breeder females, 20 breeder males, and 275 infants and juveniles, enabling a continuing annual harvest of approximately 70 animals for research; 2. To maximize the value of the animals for research purposes by offering to investigators animals that are characterized relative to the following Mamu alleles: Mamu-A*01, -A*02, -A*11, -B*01, -6*03, -B*04, and-B*17.; 3. To maximize the long term efficiency of colony production by a) establishing, managing, and utilizing a pedigree database for genetic management of the colony. The panel of 20 highly informative microsatellite polymorphisms developed in the first 5 years through other funding from NIH/NCRR, along with demographic records to establish this database, will be used to establish and verify pedigree information, manage breeding to avoid inbreeding and maintain genetic variability, while focusing on the primary goal of maximizing production and harvest schedules by utilizing data on mortality, morbidity, and fertility in the colony.