ESTABLISHMENT OF A SPF RHESUS MACAQUE COLONY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。该项目的目标是提高国家S培育印度源恒河猴(猕猴)的能力，这些恒河猴对B型疱疹病毒、SIV、SRV和STLV-1没有特定病原体(SPF)。拟建的殖民地将继续生产高质量的具有基因特征的动物，供设在西南国家灵长类动物研究中心(SNPRC)和美国西南部地区其他研究机构的NIH受资助者进行与艾滋病相关的研究。SNPRC殖民地的大部分创始股票是通过从美国空军(USAF)向灵长类中心收购一个现有的SPF殖民地获得的。向SNPRC发放了CO6赠款(RR-01-130，SPF恒河猴设施，用于SNPRC)，用于建造新住房。拟议的生产和收购计划将导致增加群体规模，以达到430只动物的稳定状态。这一目标群体规模将允许从06年开始每年收获70只动物，以满足艾滋病相关研究的关键需求和美国空军的研究需求。该殖民地生产的SPF猴子已经并将继续具有良好的血清学和遗传学特征。定期对阳性动物进行血清学检测和清除，以确保保持SPF状态。SPF群体的所有成员都进行了15个微卫星标记的基因分型，这些标记可用于评估母子关系和父子关系，并对群体进行遗传学特征描述。这些详细的遗传信息将用于积极的遗传管理计划，旨在最大限度地减少近亲繁殖，最大限度地增加遗传变异，并确保群体具有完全的血统。已经确定了每种动物的MAMU-A*01状态，并对部分群体采用了选择性育种策略，以生产携带该MHC等位基因的动物。将使用人口管理战略，通过制定最佳的生产和收获时间表，确保种群的长期生存能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to increase the nation¿s capacity to produce Indian-origin rhesus monkeys (Macaca mulatta) that are specific pathogen-free (SPF) for herpes B virus, SIV, SRV, and STLV-1. The proposed colony will continue to produce high quality genetically characterized animals for use I AIDS-related research by NIH grantees based at the Southwest National Primate Research Center (SNPRC) and at other research institutions in the southwestern region of the United States. Most of the founding stock of the SNPRC colony was obtained through acquisition of an existing SPF colony from the U.S. Air Force (USAF) to the Primate Center. A CO6 grant (RR-01-130, SPF Rhesus Macaque Facility for SNPRC) was awarded to the SNPRC for the construction of new housing. The proposed production and acquisition schedule will result in increasing the colony size to achieve a steady state of 430 animals. This target colony size will allow an annual harvesting of 70 animals per year beginning in Year 06 to fulfill critical needs in AIDS-related research and research needs of the USAF. The SPF monkeys produced by the colony have been and will continue to be well characterized serologically and genetically. Serological testing and removal of positive animals is done on a routine basis to assure maintenance of SPF status. All members of the SPF colony are genotyped for 15 microsatellite markers, which can be used to assess maternity and paternity, and to genetically characterize the colony. This detailed genetic information will be used in an active genetic management program designed to minimize inbreeding, to maximize genetic variability, and to ensure that the colony is fully pedigreed. The Mamu-A*01 status of each animal has been determined, and a selective breeding strategy has been employed with a portion of the colony to produce animals which carry this MHC allele. Demographic management strategies will be used to ensure the long-term viability of the colony through development of optimal production and harvesting schedules.