Electrophoretic Plasmonic Nanopore Genome Sequencer

 该提案的目标是开发一种方便便携的电泳等离子体纳米孔（EPN-GeneS（TM））DNA测序系统，该系统将能够在30分钟内以100美元的成本对整个人类基因组进行快速，可靠和自动化的DNA测序，这将彻底改变生物研究和医学。基本创新是基于电泳等离子体纳米孔系统（= 1.0-nm），用于在宽范围内减慢和控制单链DNA分子的移位速率，以实现准确、快速的通量、大规模并行和真实的时间单碱基对光学识别。所提出的EPN-GeneS（tm）测序仪使用具有成本效益的一次性完全集成电泳等离子体纳米孔（EPN）生物芯片，该芯片通过定制的“智能”手机连接设备进行询问，该设备集成了用于电泳流体运动控制的光流体学、标准多波长半导体激光二极管（pump & stokes）来激发易位DNA的振动模式，和DNA碱基的散射拉曼光谱特征的无标记可视化-使用集成到高分辨率手机摄像头的全息滤波器对序列进行处理，通过网络连接获取和处理序列图像，从而产生成本可承受的（100美元）DNA测序设备。在第一阶段，我们将证明控制长DNA易位率的能力，即，λ-DNA分子通过分形等离子体纳米多孔结构，使得能够使用光学成像技术对碱基对DNA序列进行真实的时间识别。在第二阶段，我们将优化和整合EPN-GeneS（TM）纳米流体生物芯片，以实现成本可承受的（100美元）高质量和可靠的完整DNA测序设备。

 DESCRIPTION (provided by applicant): The goal of this proposal is to develop a convenient and portable electrophoretic plasmonic nanopore (EPN-GeneS(tm)) DNA sequencing system that will enable the rapid, reliable, and automated DNA sequencing of an entire human genome within 30-minutes at a cost ($100) that will revolutionize biological research and medicine. The fundamental innovation is based on an electrophoretic plasmonic nanopore system (= 1.0-nm) use to slow and control the translocation rate of single stranded DNA molecules over wide ranges to enable accurate, fast throughput, massively parallel, and real time single base pair optical recognition. The propose EPN-GeneS(tm) sequencer uses a cost-effective and disposable fully integrated electrophoretic plasmonic nanopore (EPN) biochip that is interrogated via a custom "Smart" cell-phone attachment device that integrates opto-fluidics for electrophoretic fluid motion control, standard multi-wavelength semiconductor laser-diodes (pump & stokes) to excite the vibrational modes of the translocating DNA, and label-free visualization of the scattered Raman spectral signature of the DNA base-pair sequence using holographic filters integrated to the high resolution cell phone camera to acquire and process the sequence images via web connectivity, resulting in a cost affordable ($100) DNA sequencing device. In Phase I we will demonstrate the capability to control translocation rate of long DNA, i.e., Lambda-DNA, molecule through the fractal plasmonic nanoporous structures to enable the real time recognition of the base pair DNA sequence using optical imaging techniques. In Phase II we will optimize and integrate the EPN-GeneS(tm) nanofluidic biochips to achieve a cost affordable ($100) high quality and reliable complete DNA sequencing device.