Flow Cytometry

项目总结(见说明)： 核心D-流式细胞术研究核心提供集中的技术专业知识和主要仪器资源，以支持和加强HIV发病机制和治疗中需要使用流式细胞仪表型分析、细胞分类或特定免疫细胞功能评估的基础和转化性研究的实验设计和执行。一个主要目标是提供科学环境和物质资源，使初级研究人员能够将流式细胞仪技术应用于他们的项目，并鼓励已有的研究人员启动创新的先导研究。核心人员包括：Celsa Spina主任，博士，博士(ABMLI)；一名实验室经理/高级技术人员，在&gt；工作10年。有广泛的细胞学仪器使用经验(T·兰巴多)；两名技术专家，每人有20年的经验。经验(M.O‘Keefe，N.Sekiya)；初级技术员，工作4年。体验(D.Sirypangno)。主要仪器包括：三台细胞分析仪(2台激光FACS CANTO I、3台激光FACS CANTO II、2台激光iCyte LSC)和两台高速细胞分选仪(3台激光FACS Aria II、4台激光MoFlo XDP)，其中一台安装在BSL-2/3生物隔离设施内。 在核心的支持下，艾滋病毒/艾滋病研究的范围从病毒调控和致病机制的基础工作(AI038201、AI081668、AI095623、AI096113、DK035108、GM032373)到药物、疫苗和基因疗法的临床前开发(AI033292、AI055332、AI071803、AI076558、AI079031)，再到病毒传播(AI074621)、病毒潜伏期(AI080193、AI096113)、神经生物学(DA026306、MH083552、MH097520)以及病毒对实验治疗的反应(AI064086、AI087164、MH085610)的临床研究。支持和促进上述每个领域的研究：通过核心主任和技术支助人员提供关于实验设计和数据解释的专家咨询；选择最先进的仪器作为补充功能；通过专门的技术员操作员使用设备；通过既定的充值机制提供所有服务；根据个别研究项目的具体目标进行数据分析。中心与研究人员合作，开发和应用实现资助项目研究目标所需的新方法，并培训初级研究人员以最佳和正确的方式使用流式细胞术方法来解决他们的研究假设。此外，该中心还提供独特的生物隔离设施，对艾滋病毒感染者和其他有害生物样本进行活细胞分选，从而满足圣地亚哥艾滋病毒研究界的特殊需求。

PROJECT SUMMARY (See instructions): Core D - The Flow Cytometry Research Core provides a centralized resource of technical expertise and major instrumentation to support and enhance the experimental design and execution of basic and translational research in HIV pathogenesis and treatment that require use of flow cytometric phenotypic analysis, cell sorting, or evaluation of specific immune cell functions. A prime goal is to provide the scientific environment and the material resources to enable junior investigators to apply flow cytometry technology to their projects and to encourage established investigators to initiate innovative pilot studies. Core personnel include: the Director, Celsa Spina, PhD, D(ABMLI); a lab manager/senior technologist, with >10 yrs. experience using a broad range of cytometry instruments (T. Rambaldo); two technical specialists, each with >20 yrs. experience (M. O'Keefe, N. Sekiya); and a junior technician, with 4 yrs. experience (D. Sirypangno). Major instrumentation includes: three cell analyzers (2-laser FACS Canto I, 3-laser FACS Canto II, 2-laser iCyte LSC) and two high-speed cell sorters (3-laser FACS Aria II, 4-laser MoFlo XDP), one of which is housed within a BSL-2/3 biocontainment facility. The spectrum of HIV/AIDS research, supported by the Core, spans basic work on viral regulation and mechanisms of pathogenesis (AI038201, AI081668, AI095623, AI096113, DK035108, GM032373) to preclinical development of drugs, vaccines, and gene therapies (AI033292, AI055332, AI071803, AI076558, AI079031) to clinical studies of viral transmission (AI074621), viral latency (AI080193, AI096113), neurobiology (DA026306, MH083552, MH097520), and immune and viral responses to experimental treatments (AI064086, AI087164, MH085610). To support and promote research in each of these areas: expert consultation on experimental design and data interpretation is provided through the Core Director and technical support staff; state-of-the-art instrumentation is selected for complementary functions; equipment use is accessible through dedicated technician operators; all services are provided through an established recharge mechanism; and data analysis is customized to the specific objectives of individual research projects. The Core works with investigators to develop and apply new methods that are needed to attain the research goals of funded projects, and trains junior investigators to optimally and correctly use flow cytometry approaches to address their research hypotheses. In addition, the Core fills a special need of the San Diego HIV research community by providing a unique biocontainment facility that performs live cell sorting of HIV-infected and other biohazardous samples.