In Vivo Microsurgical Anastomosis of Prevascularized Tissues

描述（由申请人提供）： 工程组织血管化的两种主要方法-血管生成和血管生成-需要几天的时间来在植入的结构中产生灌注。这项拟议的R 03试点研究旨在开发第三种方法，其中将预血管化的微流体组织与宿主血管进行显微手术吻合，提供了一种直接的物理灌注途径。这项工作将使用大鼠股动脉和静脉作为受体植入内皮化的微流体胶原蛋白和纤维蛋白为基础的组织，并将分析结构中灌注和组织活力的维持。构建体将密集接种脂肪细胞以模拟软组织替代物。在这种用于血管形成的物理策略能够实现其作为血管生成和血管生成的替代方案的潜力之前，该原理验证研究是重要的必要步骤。这项工作将提供一个广泛的使能工具，用于生成血管化组织和一个具体的路径，以工程血管化脂肪组织治疗大的软组织缺损，这是经常看到肿瘤切除或重大创伤后。

DESCRIPTION (provided by applicant): The two dominant approaches to vascularization of engineered tissues-angiogenesis and vasculogenesis-require several days to generate perfusion in implanted constructs. This proposed R03 pilot study seeks to develop a third approach, in which microsurgical anastomosis of a prevascularized microfluidic tissue to host vessels provides an immediate, physical route to perfusion. This work will implant endothelialized microfluidic collagen- and fibrin-based tissues using the rat femoral artery and vein as recipients, and will analyze the maintenance of perfusion and tissue viability in the construct. The constructs will be densely seeded with adipocytes to model soft tissue replacements. This proof-of-principle study is an important requisite step before such a physical strategy for vascularization can realize its potential as an alternative to angiogenesis and vasculogenesis. This work will provide a broad enabling tool for generating vascularized tissues and a concrete path to engineering vascularized adipose tissue for treatment of large soft tissue defects, as is often seen after tumor resection or major trauma.