Cryopreservation Research/Development

冷冻保存研发计划领域通过将独特品系的小鼠的冷冻种质保存在液氮(-196℃)中，为其提供服务。超低温保存为保存不再使用的菌株提供了一种经济高效的替代方案，允许它们从货架存储中移除。此外，该实验室有44,000瓶的存储容量，作为对任何无法预测的灾难的备份；每个菌株的一些瓶保存在国家癌症研究所-弗雷德里克中央储存库。为了进一步研究癌症，冷冻生物学实验室利用最先进的技术，为保存给定的小鼠品系提供了不同的选择。这些技术包括冷冻不同发育阶段的胚胎、冷冻卵巢和冷冻精子。有了这些选择，我们能够超低温保存大多数近交系、自发突变或基因工程菌株，同时保持遗传背景和基因。

The Cryopreservation R&D program area offers services for banking unique strains of mice by preserving their frozen germplasm in liquid nitrogen (-196¿ C). Cryopreservation provides a cost-effective alternative to maintaining strains no longer in use by allowing their removal from the shelf storage. Additionally, the laboratory has a storage capacity of 44,000 vials and as a back-up against any imponderable disaster; a number of vials from each strain are kept at the National Cancer Institute-Frederick Central Repository. To further cancer research, the Cryobiology Laboratory utilizes state-of-the-art techniques that make it possible to offer different options for preserving a given mouse strain. Such techniques include freezing embryos at different developmental stages, freezing ovaries, and freezing sperm. With these options we are able to cryopreserve most inbred, spontaneous mutant, or genetically engineered strains, while maintaining the genetic background and genotype.