Transgenic Mouse Facility

弗雷德里克国家癌症研究所的转基因小鼠模型(TMM)实验室通过显微注射受精卵生产转基因小鼠，为需要研究体内基因功能的研究人员提供帮助。虽然这项服务是为每个客户的需求定制的，但这项工作的核心包括提纯要进行显微注射的DNA片段，并通过Southern印迹、聚合酶链式反应或狭缝/斑点印迹对所产生的小鼠进行分析。设计用于转基因小鼠研究的重组DNA结构的咨询是可用的。也可以在RNA或蛋白质水平上表征转基因表达的模式。转基因小鼠通常在FVB/N和C57BL/6背景下生产。

The Transgenic Mouse Model (TMM) Laboratory at the National Cancer Institute ¿ Frederick provides assistance to investigators with a need to study in vivo gene function, by producing transgenic mice by pronuclear microinjection of fertilized mouse eggs. Although this service is customized to the needs of each client, the core of this effort includes purification of the DNA fragment to be microinjected and analysis of the resulting mice by Southern blot, PCR, or slot/dot blot. Consultations for the design of recombinant DNA constructs for the use in transgenic mouse studies are available. Characterization of the pattern of transgene expression at the RNA or protein level is also available. Transgenic mice are routinely produced in FVB/N and C57BL/6 backgrounds.