Resource for Pre-Clinical Studies of AKI (Animal Models/lmaging/Renal Physiology

人类疾病的小鼠模型提供了对肾脏病理生理过程的基本见解，是测试急性肾损伤(AKI)治疗和预防方法的重要临床前资源。核心B的具体目标是提供设施和必要的技能，1)研究急性肾损伤的小鼠模型，2)小动物成像，以及3)确定急性肾损伤时的肾脏生理变化。这一核心将具体提供(1)在开发和培训使用AKI啮齿动物模型方面的专门知识，特别是在缺血/再灌注损伤、败血症和肾移植方面，(2)将提供最先进分子成像的多模式小动物成像核心，包括使用磁共振成像/光谱学的功能、结构和代谢成像、高频超声成像、显微CT、伽马射线成像(伽马相机、microSPECT/CT、microPET/CT)和光学成像(生物发光和荧光)，以及(Iii)一个生理学核心，将为研究全肾和单肾单位水平的肾功能提供专门知识和培训，包括微穿刺术和GFR的测定，肾小管液和肾小管重吸收的微量分析，肾血流动力学和肾小球反馈的评估，以及啮齿动物肾脏耗氧量的代谢评估。核心B还将提供从啮齿动物的培养中分离原代肾脏和血管细胞的技术专业知识。 Core B的目的是提供独特的资源，帮助研究人员克服障碍，利用相关的啮齿动物模型进行体内研究，并利用啮齿动物细胞系进行体外研究，以促进对AKI病理生理学的了解。Core B在支持肾脏研究界方面取得了非常成功的成果。自核心B成立以来，已经为涉及92个项目的80名首席调查员进行了5800多次程序。在80名调查员中，60人(75%)为非核心人员。每年使用Core B的调查人员数量都在增加，每年的发表率也在增加。核心B还支持了11名试点和可行性补助金获得者的研究工作。这些综合努力目前已在83份经同行审查的出版物中得到认可。先进的基础设施和核心B的独特专业知识将继续促进和UCSD之间的合作努力，并产生创新的新倡议，将推动AKI研究。

Mouse models of human disease have provided essential insights into renal pathophysiological processes and are an important preclinical resource to test therapeutic and preventive approaches in acute kidney injury (AKI). The specific aims of Core B are to provide the facilities and requisite skills 1) to study murine models of AKI, 2) for small animal imaging, and 3) to determine renal physiological changes in AKI. This core will specifically provide (i) expertise in development and training in the use of rodent models of AKI specifically in the setting of ischemia/reperfusion injury, sepsis and renal transplantation, (ii) a multi-modality small animal imaging core that will provide state-of-the-art molecular imaging, including functional, structural and metabolic imaging using magnetic resonance imaging/spectroscopy, high frequency ultrasonography, microCT, gamma-ray imaging (gamma camera, microSPECT/CT, microPET/CT), and optical imaging (bioluminescence and fluorescence), and (iii) a physiology core that will provide expertise and training for studying renal function on the whole kidney and at the single nephron level, including micropuncture techniques and determination of GFR, microanalysis of tubular fluid and tubular reabsorption, renal hemodynamics with assessment of tubuloglomerular feedback, and metabolic assessment of kidney oxygen consumption in rodents. Core B will also provide technical expertise for the isolation of primary renal and vascular cells in culture from rodents. The intent of Core B is to provide unique resources that help overcome barriers for investigators to utilize relevant rodent models for in vivo studies and rodent cell lines for in vitro studies to advance understanding of the pathophysiology of AKI. Core B has been very successful in supporting the kidney research community. Since the inception of Core B, more than 5,800 procedures have been performed for 80 principal investigators involving 92 projects. Of the 80 investigators, 60 (75%) were non-core personnel. The number of investigators using Core B each year is increasing, as is the annual publication rate. Core B has also supported the research efforts of 11 Pilot and Feasibility grant awardees. These combined efforts have been currently recognized in 83 peer-reviewed publications. The sophisticated infrastructure coupled with the unique expertise of Core B will continue to catalyze collaborative efforts between and UCSD and produce innovative new initiatives that will advance AKI research.