Request for a CyTOF Helios System

摘要 质量细胞术是一项革命性的技术，它允许多参数 用类似于流式细胞术的方式分析单细胞，但有3-4倍 维度的增加。CyTOF Helios是最先进的质量 细胞仪系统，其功能是通过适应现有的CyTOF2系统来实现的。 通过使用金属离子而不是荧光标签，CyTOF可以测量50+ 参数同时具有最小的信号重叠和信号需求 补偿。相比之下，传统的流式细胞术只能测量12-18 标记同时出现在单个细胞上。尽管我们的CyTOF2系统已经 生产高质量的数据，提高数据生产的质量和效率， 拟议的对太阳神系统的升级是实现这一目标的必要步骤 以最佳状态运行的贵重设备。这项提议寻求对 收购CyTOF Helios系统供费城退伍军人管理局使用 中心(PVAMC)。

Abstract Mass cytometry is a revolutionary technology that allows multi-parameter analysis of single cells in a manner similar to flow cytometry, but with a 3-4 fold increase in dimensionality. The CyTOF Helios is the most advanced mass cytometer system, which functions by adapting to the existing CyTOF2 system. By using metal ions rather than fluorescence tags, CyTOF can measure 50+ parameters simultaneously with minimal signal overlap and a need for signal compensation. In comparison, traditional flow cytometry can only measure 12-18 markers simultaneously on single cells. Although our CyTOF2 system is already producing high quality data, to improve the data-producing quality and efficiency, the proposed upgrade to the Helios system is a necessary step to allow this valuable equipment to function optimally. This proposal seeks support for the acquisition of CyTOF Helios system for the use at the Philadelphia VA Medical Center (PVAMC).