Tissue Core

成瘾治疗神经生物学中心 动物和组织核心摘要 Mark Ferris博士，核心主任；Paul Czoty博士，联合调查员 动物和组织核心的总体目标是生成、跟踪和分发来自 实验动物要多次进行体内和体外实验。核心将集中和标准化 啮齿动物脑组织的产生、储存和转移到项目2和项目3。这些研究包括自我 给药、病毒介导的多巴胺能D2受体基因敲除和急性/慢性治疗 项目1中确定的推定药物。通过对这些动物的繁殖负责 在项目2和项目3的研究中，核心将确保所有受试者都将在 规范的方式。大脑和周围组织将由Core组织和存储，这将确保 准确记录受试者的行为和药物史。核心组织将分发这些组织 在盲目条件下向项目2和项目3的调查人员进行成像和生化研究。小心 需要准备和处理这些样品，并精心组织信息，以确保 可以从使用这些组织样本的实验中获得可靠的结论，这使得 动物和组织核心中心是该中心的重要组成部分。具体目标是： 1.建立病毒介导的针对D2受体的基因敲除大鼠模型 项目2和项目3。 2.产生一组有可卡因自身给药史的大鼠(用药和不用药 治疗和病毒介导的基因敲除)，用于项目2和3。 3.为储存在核心内的所有组织提供一个详细记录的中心地点，并 在双盲条件下，及时将这种组织分发给项目2和项目3。 4.收集和储存将由可卡因自我给药研究产生的啮齿动物大脑 项目1，用于可能的治疗药物和其他未来潜在用途的生化分析。 5.收集和储存非人灵长类血液和脑脊液(CSF)以分析代谢物 可能的治疗药物和其他潜在的未来用途，以及存储非人类灵长类大脑样本 当项目1中的研究完成后，它们就可以使用了。 6.维护组织库，定期活检组织，确保组织完整性。

Center for the Neurobiology of Addiction Treatment Animal and Tissue Core Summary Dr. Mark Ferris, Core Director; Dr. Paul Czoty, co-investigator The overall objective of the Animal and Tissue Core is to generate, track and distribute tissue from experimental animals to multiple in vivo and in vitro experiments. The Core will centralize and standardize the generation, storage and transfer of brain tissue from rodents to Projects 2 and 3. These studies include self- administration, viral mediated gene knockdown of dopaminergic D2 receptors and acute/chronic treatment with putative medications identified in Project 1. By taking responsibility for the generation of these animals for studies in Projects 2 and 3, the Core will ensure that all subjects will be treated and euthanized in a standardized manner. Brain and peripheral tissue will be organized and stored by the Core, which will ensure accurate recording of the subjects' behavioral and pharmacological histories. The Core will distribute this tissue under blinded conditions to investigators in Projects 2 and 3 for imaging and biochemical studies. Careful preparation and handling of these samples and meticulous organization of information are required to ensure that reliable conclusions can be obtained from experiments using these tissue samples, which makes the Animal and Tissue Core an essential component of the Center. The specific goals are: 1. To generate groups of rats with viral mediated gene knockdown targeted against D2 receptors for Projects 2 and 3. 2. To generate groups of rats with a history of cocaine self-administration (with and without medication treatment and viral mediated gene knockdown) for Projects 2 and 3. 3. To provide a central locus for detailed record-keeping for all tissues stored within the Core and to provide timely distribution of this tissue under double-blinded conditions to Projects 2 and 3. 4. To collect and bank rodent brains that will be generated by cocaine self-administration studies in Project 1 for biochemical analyses of putative treatment drugs and other potential future uses. 5. To collect and bank nonhuman primate blood and cerebrospinal fluid (CSF) for analysis of metabolites of putative treatment drugs and other potential future uses, and bank nonhuman primate brain samples as they become available after studies in Project 1 are completed. 6. To maintain the tissue bank and periodically biopsy tissue to ensure tissue integrity.