Physiology

项目摘要 该愿景研究核心(VRC)的总体目标是提供： ·获得个人R01奖励范围以外的资源 ·获得单一实验室范围以外的技术专长 ·员工培训，以消除高效翻译研究和协作的障碍 VRC支持的研究领域涵盖视网膜变性的分析和治疗， 发育障碍、青光眼和其他障碍，以及一系列尖端的基本 科学倡议。我们已经实现了四个资源模块，它们延续了 这个研究小组是如何合作的，为拥有21个NEI R01奖项的15名研究人员服务。 生理学模块是一套强大的工具，供VRC教职员工用于动物模型验证和 疾病分析：ERGS、OptoMotry行为测试和一系列眼部成像工具(OCT、Mi- Cron)。我们的新模块包括体内/体外双光子成像。 具体地说，生理学模块提供： ·双UTAS ERG系统(一个在JMEC间隔室内) ·JMEC Varium内的双光电系统 ·三个微米级小鼠成像系统(一个在JMEC隔膜内) ·增加一个具有图像引导ERG功能的Micron IV小鼠成像系统 ·双光谱OCT系统(JMEC间隔室内的一个) ·一台新的Bruker Ultima双光子显微镜 ·为所有这些系统提供技术支持

Project Summary The OVERALL aims of this Vision Research Core (VRC) are to provide: · access to resources outside the scope of individual R01 awards · access to technical expertise outside the scope a single laboratory · staff training to remove barriers to efficient translational research and collaboration The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as and a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 15 investigators holding 21 NEI R01 awards. The Physiology Module is a powerful set of tools that VRC faculty use for animal model validation and disease profiling: ERGs, OptoMotry behavioral testing, and a range of ocular imaging tools (OCT, Mi- cron). Our new module includes in vivo / in vitro 2-photon imaging. Specifically the Physiology Module provides: · Dual UTAS ERG systems (one within the JMEC vivarium) · Dual OptoMotry systems within the JMEC vivarium · Three Micron mouse imaging systems (one within the JMEC vivarium) · An additional Micron IV mouse imaging system with image-guided ERG function · Dual Spectralis OCT systems (one within the JMEC vivarium) · A new Bruker Ultima 2-photon microscope · Technical support for all these systems