Platform for high throughput cell enumeration

本申请中所需的设备旨在处理样品的大量细胞枚举分析，其中感兴趣的生物体数量非常少。它包括一个歧管和塔的膜过滤，通过大量的液体样品可以通过。与传统的培养皿电镀相比，这提高了检测水平。此外，可以在膜上计数的菌落数量高于培养皿，因此需要较少的样品稀释。然后在适宜的培养基和适宜的条件下对所研究的微生物进行培养。主要申请人是一名肉类微生物学家，最近被农业和农业食品科学学院招募，以开发一个新的肉类学术和研究项目。虽然她的正式工作是动物科学系，但她的研究活动是在食品科学与营养系进行的。这个特殊的合资企业促进了来自两个部门的微生物学家之间的强有力的合作，如本应用程序所示。

The equipment required in the present application is aiming at processing numerous cell enumeration analyses for samples where the organisms of interest are in very low numbers. It consist of a manifold and towers for membrane filtration through which a large volume of liquid sample can pass. This improves the detection level compared to traditional plating in Petri dish. Also, the number of colonies that can be counted on the membrane is higher than a Petri dish so less sample dilutions are necessary. The membranes are then incubated on appropriate growth medium and under suitable conditions for the microorganism studied. The main applicant is a meat microbiologist recently recruited by the Faculty of Agriculture and Agri-Food Sciences to develop a new academic and research program in meat. Although she is officially with the Department of Animal Science, her research activities take place in the Department of Food Science and Nutrition. This particular joint venture fosters a strong collaboration between the microbiologists from the two departments as shown by this application.