Enhanced gas and temperature control for the li-cor 6400

该提案要求1.4万美元用于建造便携式气体输送设备（“GasCon”）和温度控制系统，以加强用于光合作用和呼吸研究的Li-Cor 6400气体交换系统的环境控制。GasCon将使我们能够精确地将已知O2和CO2浓度的空气输送到Li-COR 6400设备。这对于一系列需要改变O2浓度的气体交换实验和需要极低的CO2控制的实验是必要的。温度控制系统包括一个高精度，冷冻水浴和两个热控制块，将扩展Li-6400的热控制到5°C和45°C之间。这将使我们能够评估光合作用在生理相关范围内的温度响应。水浴还将为气体交换设备的湿度校准和植物水分状况的湿度计评估提供精确的温度控制。该设备将是便携式的，可以在远离我在多伦多大学的实验室的地方使用。有了它，我的实验室将能够进行基于发现的研究，研究决定植物对气候变化和大气二氧化碳浓度反应的生理机制。

The proposal requests $14,000 to build a portable, gas delivery apparatus (The "GasCon"), and a temperature control system to enhance the environmental control of the Li-Cor 6400 gas exchange system used for photosynthesis and respiration studies.  The GasCon will allow us to precisely deliver air of known O2 and CO2 concentration to the Li-COR 6400 apparatus.  This is necessary for a range of gas exchange experiments where we need to vary the concentration of O2, and for experiments where extremely low CO2 control is required.  The temperature control system consists of a high-precision, refrigerated water bath and two thermal control blocks that will extend the thermal control by the Li-6400 to between 5°C and 45°C. This will allow us to evaluate the temperature response of photosynthesis across the physiologically relevant range. The water bath will also provide the precise temperature control needed for humidity calibration of gas exchange equipment, and psychrometric assessments of plant water status.  The equipment will be portable, enabling use in locations away from my lab at the University of Toronto. With it, my lab will be able to pursue discovery-based research into the physiological mechanisms that determine the response of plants to climate change and atmospheric CO2 enrichment.