Microplate spectrophotometer for reporter gene assays

拟南芥是农业生物技术的一种模式生物，它为了解基因功能如何影响作物改良提供了机会。随着拟南芥基因组测序计划的完成，下一个主要挑战是基因功能的确定。报告基因检测是研究基因功能最有效的方法之一，也是必不可少的组成部分。越来越多的报告基因检测是基于荧光或发光检测。该检测可以在微孔板分光光度计中轻松有效地实现，该分光光度计可以读取6至384孔格式的荧光或发光，允许高通量测量基因表达，激活，抑制和蛋白质降解。申请资金用于购买BioTek Synergy HT多重检测微孔板分光光度计（总费用41,708.00美元）。该设备将广泛用于NSERC支持的研究项目。这种微孔板分光光度计的获得将为nserc支持的研究项目的成功提供特殊的机会，并且对于在机械水平上确定植物中大量基因的功能至关重要。此外，该设备将提供给不列颠哥伦比亚省的植物生物界，从而大规模地促进科学的进步和卓越。

Arabidopsis is a model organism for agricultural biotechnology, providing the opportunity to get key insights into the way that gene function can affect crop improvement. With the completion of the Arabidopsis genome sequencing project, the next major challenge is the determination of gene function. Reporter gene assays are one of the most efficient applications and essential components for the studies of gene functions. More and more reporter gene assays are based on either fluorometric or luminometric detection. The assays can be easily and efficiently achieved in a Microplate Spectrophotometer, which could read fluorescence or luminescence in 6- to 384-well formats, allowing high throughput measurements of gene expression, activation, repression, and protein degradation. Funds are requested for purchasing a BioTek Synergy HT Multi-Detection Microplate Spectrophotometer (total cost $41,708.00). This equipment will be extensively used for the research programs supported by the NSERC. The acquisition of such a Microplate Spectrophotometer will provide exceptional opportunities for the success of the NSERC-supported research programs, and is essential for determining the function of large number of genes in plants at mechanistic level. Furthermore, this equipment will be made available for the plant biology community in British Columbia, thereby promoting advance and excellence in science in a large scale.