Bio-rad PDS-1000/He particle delivery system for transformation of single-cell C4 photosynthesis species

我们对生物如何在分子水平上发挥作用的理解最近取得了进展，这是通过基因操作取得的。这一科学学科在确定基因的结构和功能以及它们在健康和农业中的用途方面做出了重大贡献。然而，将遗传物质引入感兴趣的细胞和组织仍然是许多领域分子研究的限制步骤。在这项RTI赠款申请中，申请资金用于购买Bio-Rad PDS-1000/He粒子输送系统(25,278美元)，以及HEPTA适配器升级模块(5,720美元)。我提议的研究需要一种生物颗粒递送系统来检查能够在单个细胞中进行C4光合作用的新植物系统中的基因功能。目前，滑铁卢大学还没有提供这种仪器：a)除了我的研究项目，它的存在也将极大地有利于其他实验室，b)它将是一种可访问和维护良好的通用设备，可用于高素质人员的研究和教学，c)购买该设备将使研究人员有机会转化许多不同类型的细胞，d)提高滑铁卢大学的性能水平。

Recent advances in our understanding of how biological organisms function at the molecular level are made through genetic manipulation. This scientific discipline has contributed significantly to identify the structure and function of genes and their uses in health and agriculture. Yet the introduction of genetic material into cells and tissues of interest remains a limiting step for molecular investigations in many fields. In this RTI grant application, funds are requested for the purchase of a Bio-Rad PDS-1000/He Particle Delivery system ($25,278) with a Hepta Adaptor Upgrade module ($5,720). My proposed research requires a biolistic particle delivery system to examine gene function in novel plant systems capable of performing C4 photosynthesis in individual cells. Currently, this instrument is not available at the University of Waterloo: a) In addition to my research program, its presence would also greatly be beneficial for other laboratories, b) It would be an accessible and well-maintained common equipment available for research and teaching of highly qualified personnel, c) Purchase of this equipment will allow researchers with opportunity to transform many different cell types, d) Increase the performance level at the University of Waterloo.