Biochemistry analyzer for the modeling and control of mammalian cell culture processes

这项研究计划旨在购置一台能够快速、同时测量哺乳动物细胞培养物中关键代谢物的生化分析仪。请求提供的设备主要用于支持侧重于改进哺乳动物细胞培养过程的开发和控制的研究。哺乳动物细胞越来越多地用于制造高价值的生物制药产品，如单克隆抗体、病毒疫苗和激素。对大批量产品不断增长的需求推动了提高细胞培养过程的生产率和成本效率的需求。尽管该领域取得了进展，但在实现所需产品质量和提高产量方面仍然存在重大挑战。拟议的研究旨在了解和表征细胞代谢和生理学，作为开发和控制细胞培养过程的基础。该方法在很大程度上依赖于灵敏和快速测量重要生物过程变量的能力。通过解决未满足的量化需求，分析仪将对研究项目的实现至关重要。这项研究预计将为动物细胞技术领域做出重大贡献，并对所有操作规模的生物工艺的发展产生影响，从临床前研究所需的少量到批量生产。这一研究领域的进展将有助于消除生物治疗发展的瓶颈，从而对加拿大人的健康和加拿大生物制药业产生积极影响。该仪器还因其支持Ecole Polytechnique在组织工程和生物医学等有前途的领域正在进行的和新兴的哺乳动物细胞培养研究活动的潜力而被选中。它的可用性也将通过为学生提供最先进设备的实践经验对学生的培训产生积极影响。

This research proposal aims at the acquisition of a biochemistry analyzer capable of providing rapid and simultaneous measurements of key metabolites in mammalian cell cultures. The requested equipment is intended primarily to support research focused on improving the development and control of mammalian cell culture processes. Mammalian cells are increasingly being used for the manufacture of high-value biopharmaceuticals products such as monoclonal antibodies, viral vaccines and hormones. The growing demand for high volume products is driving the need to improve the productivity and cost efficiency of cell culture processes. Despite progress in the field, significant challenges remain to achieve desired product quality and increase the yields. The proposed research aims at understanding and characterising the cellular metabolism and physiology, as a basis for the development and control of cell culture processes. The methodology relies heavily on the ability to sensitively and rapidly measure important bioprocess variables. By addressing unmet quantification needs, the analyzer will be crucial for the realization of the research project. This research is expected to bring a significant contribution to the field of animal cell technology and have an impact on the development of bioprocesses at all scales of operation, from the small amounts required for preclinical studies through to bulk manufacture. Advances in this area of research will contribute to help eliminating the bottleneck in the development of biotherapeutics, thus impacting positively on the health of Canadians and the Canadian biopharmaceutical industry. The instrument was also selected for its potential to support on-going and new emerging mammalian cell culture research activities at Ecole Polytechnique in promising areas such as tissue engineering and biomedecine. Its availability will also positively impact the training of students by providing them with hands-on experience on state-of-the-art equipment.