Anaerobic cabinet

本研究和工具提案描述了替换Ryerson陈旧的厌氧手套箱/箱所需的设备。退役的橱柜是校园唯一的厌氧系统，该系统的不可用直接影响到现有的研究项目和对应用和环境微生物学的支持；这是瑞尔森大学生物科学的主要研究领域。例如，厌氧箱将成为与加拿大环境界面和生物膜主席相关的研究生的核心设备，他们将研究厌氧纤维素分解细菌，作为现有研究合作的一部分。该项目部分由工业界资助，其总体目标是开发技术，使纤维素生物质（如草和木材）转化为乙醇生产具有成本效益。另一名学生和博士后参与了一个研究含水层中微生物与污染物（非水相液体，NAPL）相互作用的合作项目。该项目需要厌氧设施来研究模拟地下水和地下环境条件的模型系统中的生物膜发育和微生物群落行为。与工程系统中微生物絮凝体和结构研究相关的学生需要厌氧设施来研究生物膜的发展，这是工业资助和NSERC战略资助项目的一部分，重点是厌氧膜生物反应器的发展，用于废水和固体处理的能源生产。化学与生物系在环境科学方面经历了显著的增长，包括最近在环境微生物学方面的两个任命（2006年和2007年），以及2006年秋季引入的新的分子科学研究生课程。因此，迫切需要升级我们的厌氧培养设备，以支持我们目前的研究计划和新教师的需求。

This Research and Tools proposal describes equipment required to replace an obsolete anaerobic glove box/cabinet at Ryerson. The decommissioned cabinet has been the only anaerobic system on campus and the unavailability of such system directly affects existing research projects and support for applied and environmental microbiology; a major area of activity in the biological sciences at Ryerson. For example, the anaerobic cabinet will be the core equipment for graduate students associated with the Canada Chair in Environmental Interfaces and Biofilms who will study anaerobic cellulolytic bacteria as part of an existing research collaboration. This project is partly funded by industry with the overall goal of technology development enabling cost effective bioconversion of cellulosic biomass such as grass and wood for ethanol production. Another student and postdoctoral fellow are involved in a collaboration focusing on microbial-contaminant (nonaqueous phase liquids, NAPL) interactions in aquifers. Anaerobic facilities are needed for that project to study biofilm development and microbial community behavior in model systems that simulate the conditions in groundwater and subsurface environments. Students associated with the research on microbial flocs and structures in engineered systems require anaerobic facilities to study biofilm development as part of industry-funded and NSERC Strategic Grant projects that focus on the development of anaerobic membrane bioreactors to be applied to wastewater and solids treatment for energy production. The Department of Chemistry & Biology experienced notable growth in environmental sciences, including two recent appointments (2006 and 2007) in environmental microbiology, as well as the new Molecular Sciences graduate program introduced in fall 2006. Thus, there is an immediate need to upgrade our anaerobic culture equipment to support our current research program and the needs of new faculty.