A visualized patch clamp recording system

一个可视化的膜片钳记录系统是需要扩展我目前的研究计划对中央听觉系统（NSERC发现基金支持）。该设备将使我们能够进行听觉中脑细胞生理学的研究。可视化膜片钳记录方法具有盲记录的优点，允许详细调查的离子通道和提高分辨率的生理事件的信号处理。可视化膜片钳记录方法对于研究发育中的神经元（例如幼年动物听觉脑干中的神经元）尤其必要。这些神经元太小或太脆弱，无法用尖电极记录或盲膜片钳方法刺穿。将使用可视化膜片钳记录系统实现稳定记录。然后可以应用各种药理学试剂来研究听觉神经元的特定突触受体和离子通道，以及荧光染料来检查被记录的神经元的形态和位置。 我请求拨款购买必要的设备，包括显微镜，显微操作器和渗透压计，为可视化膜片钳记录系统。利用该系统，我们将在细胞和分子水平上研究听觉神经元的信息处理、可塑性、发育和功能障碍的神经机制。在我的实验室开发可视化膜片钳记录系统将是非常有价值的听觉领域，为未来的研究提供了一个新的方向，并确保我们的实验室保持在听觉神经科学的前沿。这项设备赠款将使听觉神经生理学的进步成为可能，这将对加拿大神经科学大有裨益。拨款申请是紧迫的，因为我的小组中的几个研究生需要一个可视化的膜片钳记录系统，以确保高质量的记录和可靠的结果，为拟议的研究。

A visualized patch clamp recording system is needed to extend my current program of research on the central auditory system (NSERC Discovery Grant supported). The equipment will allow us to carry out the investigation of cellular physiology of the auditory midbrain. The visualized patch clamp recording method has the advantage over blind recordings of allowing detailed investigation of ion channels and improved resolution of physiological events underlying signal processing. The visualized patch clamp recording method is especially necessary for studying developing neurons, such as those in the auditory brainstem of young animals. These neurons are too small or fragile to impale using a sharp electrode recording or a blind patch clamp method. Stable recordings will be achieved with a visualized patch clamp recording system. It is then possible to apply various pharmacological agents to investigate specific synaptic receptors and ion channels of auditory neurons, and fluorescent dyes to examine the morphology and location of the neuron being recorded.   I am requesting the grant money to purchase essential equipment, including a microscope, a micromanipulator and an osmometer, for a visualized patch clamp recording system. With this system we will study neuronal mechanisms of information processing, plasticity, development, and dysfunction of auditory neurons at cellular and molecular levels. Development of a visualized patch clamp recording system in my laboratory would be greatly valuable to the auditory field, providing a novel direction for future research and ensuring that our laboratory remains on the cutting edge of auditory neuroscience. The advances in auditory neurophysiology that will be made possible by this equipment grant will be of great benefit to Canadian neuroscience. The grant request is urgent as several graduate students in my group require a visualized patch clamp recording system to ensure high quality recordings and reliable results for the proposed research.