Temperature-controllable and oxygen-free french press system

温度可控且无氧的French Press系统能够在厌氧条件下非侵入性地快速破坏微生物细胞。 与其他方法如超声处理（~50%）相比，它几乎完全破坏小细胞（一次通过>90%）。目前，滑铁卢大学生物系还没有这样的细胞破碎系统。 我们建议购买一个温度可控和无氧的法国新闻系统，以帮助纯化和表征氧敏感酶的超嗜热微生物和酵母细胞。 该系统也可用于其他类型的细胞，如细菌和其他真核细胞，并且根据需要和细胞可用性，它可以处理1至35 ml的样品体积。拟议的温度可控和无氧法国新闻系统将加快氧敏感酶样品制备程序，从而更有效地利用资源和减少培训时间，并将加快研究进展，从而提高我们在滑铁卢大学的培训计划的质量。

A temperature-controllable and oxygen-free French Press system is capable of rapidly disrupting microbial cells non-invasively under anaerobic conditions.  It provides a nearly complete breakage of small cells (>90% with one pass) compared to other methods such as sonication (~50%). Currently, there is no such system for cell disruption in the Department of Biology at the University of Waterloo.  We propose to purchase a temperature-controllable and oxygen-free French Press system to aid in the purification and characterization of oxygen-sensitive enzymes from hyperthermophilic microorganisms and yeast cells.  This system can also be used for other types of cells, such as bacterial and other eukaryotic cells, and it can handle sample-volumes from 1 to 35 ml depending on the need and cell availability. The proposed temperature-controllable and oxygen-free French Press system will speed up oxygen-sensitive enzyme sample preparation procedures, resulting in more efficient use of resources and reduced training time, and will accelerate research progress, thus increasing quality of our training programs at the University of Waterloo.