Microplate reader

微量板读数是一种基本的实验室仪器，用于检测微滴定板中的生物和化学事件。微滴定板是由多根试管组成的阵列，每根试管内含有少量样品。在微板阅读器中，高强度光源照亮每个样品，每个样品发出的光由探测器进行量化。最有用的检测模式是吸光度、发光、荧光强度和偏振。为了详细研究生化反应，还需要具备时间分辨、温度调节和震动的能力。由于有这么多不同的荧光团和发色团可用，阅读器也必须是可调的，并能够收集发射光谱。需要连接到微孔板读取器的计算机来收集和分析所收集的大量数据。所有生物实验室研究人员都需要使用微板阅读器进行基本分析(例如，酶联免疫吸附分析--ELISA；监测细胞生长；量化蛋白质和核酸浓度)，并经常用于更复杂的蛋白质-蛋白质和其他分子相互作用。因此，该仪器是任何生物学研究计划的重要组成部分。

Microplate readers are basic laboratory instruments designed to detect biological and chemical events in microtiter plates. Microtiter plates are arrays of "test tubes", each containing a small volume of sample.  In the microplate reader, a high intensity light source illuminates each sample, and the light emitted from each sample is quantified by a detector. The most useful detection modes are absorbance, luminescence, fluorescence intensity and polarisation. In order to investigate biochemical reactions in detail, capabilities for time-resolution, temperature adjustment, and shaking are also required. Since so many different fluorophores and chromophores are available, the reader must also be tunable and enable collection of emitted spectra. Computers interfaced to the microplate reader are required to collect and analyse the large amount of data that is collected. All biological laboratory researchers require access to a microplate reader for basic assays (e.g., the enzyme-linked immunosorbent assay - ELISA; monitoring cell growth; quantifying protein and nucleic acid concentrations) and often for more complex protein-protein and other molecular interactions. This instrument is thus an essential component of any biological research program.