Biosafety cabinet for surface modification of quantum dots to preconcentrate trace prion protein selectively from biological fluids

我的研究计划将继续开发亲和力探针，特别是分子印迹聚合物(MIP)，用于改善人类健康和动物疾病的早期诊断的生物医学应用。与可传播海绵状脑病(TSE)相关的异常普恩蛋白(PrPSc)可能对全球食品安全和公共健康构成严重且可证明的风险。通过使用定制的MIP的分子识别特性选择性预浓缩，在超级竞争水平上测定PrPSc是最有希望的。需要在MIP上选择性预浓缩后同时测定几种异常蛋白质。毛细管电泳和微流控可以提供许多分析分离优点。我们的短期目标是制备新型的MIP纳米球，它可以专门识别具有生物医学意义的单一目标蛋白质。分析科学中的一些关键的当代和新出现的挑战将通过先进的仪器方法使用基于MIP纳米球的方法来解决。我们的长期目标是升级基于MIP纳米球的强大设备、仪器、方法、设施和专业知识的开发，有朝一日将与渥太华和加拿大各地的科学部门、政府实验室和研究医院合作，为生物医学研究做出贡献。

My research program will continue with the development of affinity probes, especially molecularly imprinted polymers (MIPs), in biomedical applications for both the improvement to human health care and the early diagnosis of animal diseases.  Abnormal prion protein (PrPSc) associated with transmissible spongiform encephalopathies (TSEs) probably present a serious and demonstrable risk to food safety and public health worldwide.  The determination of PrPSc at ultratrace levels is most promising by selective pre-concentration using the molecular recognition property of a tailor-made MIP.  For the determination of several abnormal proteins simultaneously after selective pre-concentration on MIPs, capillary electrophoresis and microfluidics can offer many analytical separation merits.  Our short-term objective is focused on the preparation of novel MIP nanospheres which can specifically recognize single target proteins of biomedical significance.  Key contemporary and emerging challenges in analytical sciences will be tackled using MIP nanospheres-based methods in an advanced instrumental approach.  Our long-term objective is escalating the development of powerful MIP nanospheres-based devices, instruments, methods, facilities and expertise that will one day contribute to biomedical research in collaboration with science departments, government laboratories and research hospitals in Ottawa and across Canada.