Digital micromirror applied to laminar optical tomography

小动物漫射光学和分子成像的最新进展和商业产品的增加为分子生物学社区提供了显著的好处。内源性血液造影剂已被用于神经元激活和血管室的成像。例如，固有成像（反射测量）可用于监测开放皮层表面（100-400 μ m深度）的神经元激活，具有高图像分辨率（10 μ m平面分辨率）。由于光传播的漫射特性和与相机相比的离散测量次数，基于光纤的系统可以以空间分辨率为代价对更深的结构进行成像。此外，由于组织的非均匀吸收和散射特性，目前的技术缺乏定量。本提案旨在通过开发一种基于数字微镜的新型快速成像仪来解决其中的一些问题，该成像仪优化了空间分辨率，深度可达8mm。该想法源于最近在3D层流光学断层扫描（LOD）上所做的工作，其中高密度的照明和检测位置用于高速扫描组织，并结合漫射光学断层扫描以更高分辨率恢复图像。在这里，我们建议通过使用数字微镜来产生特定的光模式和检测来进一步开展这项工作，以进一步重建图像。

Recent progress and increased commercial offerings for diffuse optical and molecular imaging for small animals has provided significant benefits to the molecular biology community. Endogenous contrast of blood has been used to image neuronal activation and vascular compartments. For example intrinsic imaging (reflectometry) can be used to monitor neuronal activation at the surface of the open cortex (100-400 µm depth) with high image resolution (10 µm plane resolution). Fibre based systems can image deeper structures at the price of spatial resolution due to the diffuse nature of light propagation and the discrete number of measurements taken when compared to a camera. Moreover present techniques lack quantification due to the heterogeneous absorption and scattering properties of tissues.The present proposal aims to resolve some of these issues by developing a new fast imager based on digital micro-mirrors optimized for spatial resolution up to depths reaching 8mm. The idea follows on recent work done on 3D Laminar Optical Tomography (LOD) where a high density combination of illumination and detection positions were used to scan tissues at high speed in combination with diffuse optical tomography to recover images at higher resolution. Here we propose to further this work by using digital micro-mirrors to generate specific patterns of light and detection to further the image reconstruction.