Towards structure determination in membrane proteins: fast MAS probe for sensitivity enhancement through direct proton detection

固态核磁共振（NMR）作为肽和蛋白质结构测定的有力工具正在迅速发展，这是了解其功能的关键。特别感兴趣的是膜肽和蛋白质，它们不容易通过其他高分辨率技术如X射线晶体学和溶液状态NMR进行结构分析。因此，固态NMR是唯一可以提供这些系统中结构细节的明确信息的技术。 该提案中概述的研究重点是开发新的固态NMR方法用于结构研究，旨在提高测量的灵敏度，以及这些方法在感兴趣的生物系统中的应用，如膜和细胞色素相关的髓鞘碱性蛋白和蛋白质视紫质。

Solid-state nuclear magnetic resonance (NMR) is rapidly advancing as a powerful tool for structure determination in peptides and proteins, a key to the understanding of their functions. Of particular interest are membrane peptides and proteins that are not readily amenable to structural analysis by other high-resolution techniques such as X-ray crystallography and solution state NMR. Thus, solid-state NMR is the only technique that can provide definitive information on the structural detail in these systems.  The research outlined in this proposal focuses on the development of new solid-state NMR methods for structural studies aiming at the increase of sensitivity of measurements, and on the applications of these methods in biological systems of interest, such as membrane- and cytoskeleton-associated myelin basic protein, and proteorhodopsin.