Structural investigations of protein self-assembly

我的研究目标是确定蛋白质如何结合联合收割机形成复杂的分子机器，以及蛋白质如何在整个细胞中传递化学信号。我提出的研究计划分为三个主要项目：第一个项目研究自组装蛋白ClpX及其在降解或去除细胞中受损蛋白质中的作用。第二个项目研究蛋白SOX 9以不同方式结合DNA的能力。SOX9是男性性别决定的必需蛋白质。第三个项目的重点是原子水平，结构决定蛋白质参与噬菌体病毒组装。所有拟议的项目都结合了分子生物学和生物化学技术，包括核磁共振光谱学。

The objective of my research is to determine how proteins combine to form complex molecular machines and how proteins transmit chemical signals throughout the cell. My proposed research program is divided into three major projects: The first project studies the self-assembly the protein ClpX and its role in the degradation, or removal, of damaged proteins in the cell. The second project studies the ability of the protein SOX9 to bind DNA in different ways. SOX9 is an essential protein in male sex determination. The third project focuses upon the atomic level, structural determination of proteins involved in bacteriophage viral assembly. All of the proposed projects draw upon a combination of molecular biological and biochemical techniques including nuclear magnetic resonance spectroscopy.