Use of eukaryotic algae to deliver inexpensive human proteins for the treatment of Type 1 diabetes

这项提议旨在开发一种强大的新表达系统，以生产和输送廉价和高质量的人类治疗性蛋白，用于治疗1型糖尿病。新的表达系统是基于绿藻的叶绿体转化利用。叶绿体是绿藻和植物细胞中发现的一种膜结合细胞器，负责进行光合作用。在绿藻和高等植物中，每个细胞都有许多拷贝的叶绿体DNA，因此，由于转化DNA的高拷贝数扩增，叶绿体转化具有产生大量外源蛋白的潜力。

This proposal aims to develop a powerful new expression system to produce and deliver inexpensive and high quantities of human therapeutic proteins for the treatment of Type 1 diabetes. The new expression system is based on the utilization of green algae by means of chloroplast transformation. The chloroplast is a membrane-bound organelle found in green algae and plant cells that conducts photosynthesis. There are many copies of chloroplast DNA per cell in both green algae and higher plants, and therefore chloroplast transformation has the potential to generate large amounts of foreign proteins due to the high copy number amplification of the transforming DNA.