Plant tissue culture chamber for in vitro regenration of single-cell c4 photosynthesis species

农艺性状和作物产量的植物表现的改良通常通过传统的植物育种方法进行。 然而，作物改良的最新进展是通过涉及遗传操作的生物技术实现的。尽管植物生物技术在遗传改良中的应用极大地促进了粮食产量和人类健康，但在许多植物物种中，将遗传物质引入感兴趣的细胞和组织中仍然是一项具有挑战性的任务，主要是由于体外再生效率低。在RTI的赠款申请中，要求提供资金用于购买Percival组织培养室（19，440美元），该培养室经过改装以提供高光强。我的研究计划需要一个可靠的腔室，以建立一个有效的体外再生协议所需的可转化系统，以检查新的植物物种的基因功能，能够在单个细胞中进行C4光合作用。

Improvement of plant performance for agronomic traits and crop yield was generally carried out by traditional plant breeding methods. However, recent advances in crop improvement are accomplished through biotechnological techniques involved genetic manipulation. Although, this application of plant biotechnology for the genetic improvement has greatly contributed to increase food production and human health, the introduction of genetic material into cells and tissues of interest remains a challenging task in many plant species mainly due to low in vitro regeneration efficiency. In this RTI grant application, funds are requested for the purchase of a Percival tissue culture chamber ($19,440) modified to provide high light intensity. My research program requires a reliable chamber to establish an efficient in vitro regeneration protocol necessary for a transformable system to examine gene function in novel plant species capable of performing C4 photosynthesis in individual cells.