HPLC sytem for analysis of lipids, carbohydrates and other bioactives

为支持达尔豪西大学六名教职员工和他们的研究团队的计划，需要一套高效液相色谱(HPLC)系统。所要求的配置包括蒸发光散射检测器(ELSD)，这是众所周知的通用检测器，因为它能够检测所有分子，而不考虑结构或电荷。它特别适合于用通常配置的仪器无法检测到的化合物，如脂类。该高效液相色谱法的主要应用将是在脂类分析中，特别是在完整的脂类结构的表征和分离中。这种类脂分析将支持多个领域的研究，包括食物网阐明、调查物理性质和结构之间的关系，以及确定替代燃料来源。在确定从动物和鱼类加工废物中回收的一系列生物活性物质，包括脂溶营养食品、酶和胶原蛋白方面，它也是必不可少的。高效液相系统还将用于营养分析，以调查营养胁迫对细菌，特别是李斯特菌生存的影响。最后，发酵和生物修复研究中的碳水化合物和碳氢化合物水平将使用该设备进行测定。因此，这里要求的高效液相系统将使六个申请者的不同研究计划取得重大进展。

A high performance liquid chromatography (HPLC) system is requested to support the programs of six faculty members and their research teams at Dalhousie. The requested configuration incorporates an evaporative light scattering detector (ELSD) which is known as a universal detector because of its ability to detect all molecules regardless of structure or charge. It is particularly appropriate for compounds such as lipids that cannot be detected with the typically configured instruments. The primary application of this HPLC will be in the analysis of lipids, specifically in the characterization and separation of intact lipid structures. Such lipid analysis will support research in a number of areas, including food web elucidation, investigating relationships between physical properties and structure, and identifying alternative fuel sources. It will also be essential in determining a range of bioactives recovered from animal and fish processing waste, including lipid-soluble nutraceuticals, enzymes and collagen. The HPLC system will also be used in the analysis of nutrients to investigate the impact of nutrient stress on survival of bacteria, specifically Listeria sp. Lastly, carbohydrate and hydrocarbon levels in fermentation and bioremediation studies will be determined using this equipment. Thus, the HPLC system requested here will enable significant advancements to be made in the diverse research programs of the six applicants.