Technology development for proteome analysis incorporating separations and mass spectrometry

随着分析化学的技术进步，生物科学中的许多重大进步为样品分析提供了新的机会。蛋白质组学就是一个完美的例子，质谱学、分离科学和计算分析的进步使蛋白质的全面表征成为可能。通过这样做，人们基本上可以获得一个有机体的“分子”描述，从而提供对生物途径的更好的理解，帮助药物设计，还可以提供识别有机体特定状态的分子指纹(又名。疾病生物标志物)。随着新技术的出现，蛋白质组分析面临的挑战也在不断演变。蛋白质组学曾经是一个电离和解释质谱图的问题，现在(除其他方面外)将重点放在进一步增加样本吞吐量上，以实现对临床上有意义的样本大小的分析。蛋白质修饰的表征、蛋白质表达变化的量化以及质谱学数据的验证也继续是该领域持续研究的主题。

Many significant advancements in biological science have followed technological advancements in analytical chemistry, which afford new opportunities for sample analysis. Proteomics is a perfect example of this, where advances in mass spectrometry, separation science, and computational analysis have permitted the comprehensive characterization of proteins. In doing so, one can essentially obtain a 'molecular' description of an organism, and therefore provide an improved understanding of biological pathways, assist in drug design, and also provide molecular fingerprints which identify a particular state of an organism (a.k.a. disease biomarkers). Current challenges in proteome analysis continue to evolve, as new technologies are created. What was once a problem of ionization and interpretation of mass spectrometry patterns, proteomics now focuses (among other aspects) on further increasing sample throughput to enable the analysis of clinically significant sample sizes. The characterization of protein modifications, quantitation of changing protein expression, and the validation of mass spectral data also continue to be topics of continued research in this field.