Flavonol deglycosylation in arabidopsis

基因组测序和代谢物分析技术的可用性为科学家研究植物的次生代谢提供了必要的工具。在植物中，黄酮类化合物的积累受发育和环境胁迫因素的调控。在许多植物中，黄酮醇苷缀合物是类黄酮生物合成的主要终产物和储存库。有趣的是，解共轭黄酮醇苷元库在植物的生理决定过程中增加。虽然有许多研究描述了促进黄酮醇生物合成的基因和酶，但关于黄酮醇去糖基化和转化的生化过程的信息很少。在过去的几十年里，越来越多的证据表明，糖基水解酶介导从糖苷缀合的类黄酮中释放生物活性苷元。拟南芥已成为黄酮类化合物生物化学和遗传学的模型。我们将分析和描述在植物发育和环境胁迫驯化过程中黄酮醇苷向苷元池转化的机制。

The availability of sequenced genomes and metabolite profiling technologies offer scientists the required tools to investigate secondary metabolism in plants. In plants, flavonoid accumulation is regulated by developmental and environmental stress factors. In many plants, flavonol glycoside conjugates accumulate as major end products and storage pools of flavonoid biosynthesis. Interestingly, the deconjugated flavonol aglycone pool is increased during physiologically determined processes in plants. Although, there are numerous studies describing the genes and enzymes promoting flavonol biosynthesis, very little information exists on the biochemical processes that govern flavonol deglycosylation and turnover. Over the past few decades, there is mounting evidence that glycosyl hydrolases mediate the liberation of bioactive aglycones from glycoside conjugated flavonoids. Arabidopsis thaliana has become a model for flavonoid biochemistry and genetics. We will analyze and describe the mechanisms involved in the conversion of flavonol glycoside to aglycone pools during plant development and environmental stress acclimation.