Investigation of practices and technologies for lion's mane production, an emerging medicinal mushroom for Canada

加拿大新兴药用蘑菇狮鬃菇生产实践和技术的调查

• 批准号: 570913-2021
• 负责人: Bozzo, Gale
• 金额: $ 3.67万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=722531
• 关键词: Investigation; practices; technologies; lion; mane

Lion's mane (Hericum erinaceus) is an edible mushroom that is widely used in for traditional medicine in China. In North America, there has been relatively little focus on lion's mane. The therapeutic effects of lion's mane mushroom extracts have been tested in animal models and human cell lines, and positive benefits include stimulation of nerve growth factor production. The fruiting bodies and mycelia of lion's mane and related Hericium species respectively contain hericenones and erinacines, both of which are postulated as nerve growth factor biosynthesis stimulants. To feasibly produce lion's mane and other medicinal basidiomycetes more information is needed on how their cultivation is impacted by production-related factors. There is widespread knowledge that the type of culture media affects the growth dynamics of lion's mane mycelia, but not much is known about the impact on erinacine profiles. Our research project is focused on developing optimal practices and/or technologies for the cultivation of lion's mane mycelia as well as those of other Hericium species. We will develop a cultivation strategy that incorporates the best culture media type and temporary immersion regimen for cultivation of high erinacine mycelia in liquid culture. In addition, we will explore the impact of exposing mycelia to light emitting diodes on whether fruiting and hericenone content of Hericium mushrooms are improved relative to cultivation under darkness. We will verify the nutraceutical potential of extracts prepared from mycelia and fruiting bodies of various Hericium species cultivated under optimal production practices by testing their impact on neuronal model systems. The proposed research will provide unique training opportunities for 2 MSc students in multidisciplinary research, including mycology, analytical chemistry, biochemistry, electrophysiology, pharmacology and pharmacogenetic skills that are in high demand within the scientific, nutraceutical and mushroom science sectors. The research will provide ready to use practices/technologies for the adoption of lion's mane mushroom cultivation by Canadian mushroom growers as well as the development of products for the burgeoning nutraceutical sector in Canada.

狮子的鬃毛（Hericum erinaceus）是一种可食用的蘑菇，在中国广泛用于传统医学。在北美，对狮子的鬃毛的关注较少。狮子蘑菇提取物的治疗作用已在动物模型和人类细胞系中进行了测试，积极的好处包括刺激神经生长因子的产生。狮子鬃毛和相关的印第安植物物种的结实体和菌丝体分别包含黑白酮和erinacines，这两种都被认为是神经生长因子生物合成刺激剂。为了使狮子的鬃毛和其他医学基础菌co虫可行，需要更多有关其培养如何受到与生产有关的因素影响的信息。人们普遍认为，培养基的类型会影响狮子鬃毛菌丝体的生长动态，但对对Erinacine概况的影响知之甚少。我们的研究项目侧重于开发最佳实践和/或技术，以培养狮子的鬃毛菌丝体以及其他文化植物的最佳实践和/或技术。我们将制定一种培养策略，该策略结合了最佳培养基类型和临时浸入疗法，用于在液体培养中种植高艾米胺。此外，我们将探讨将菌丝体暴露于发光二极管上的影响，以相对于黑暗中的培养，地印第安蘑菇的果实和印第安蘑菇含量是否得到改善。我们将通过测试其对神经元模型系统的影响，从最佳生产实践中种植的各种Hericium种类的菌丝体和果实物体中制备的提取物的营养潜力。拟议的研究将为2位MSC学生提供多学科研究的独特培训机会，包括真菌学，分析化学，生物化学，电生理学，药理学和药物遗传学技能，这些技能在科学，营养和蘑菇科学领域的需求很高。这项研究将为加拿大蘑菇种植者以及加拿大迅速发展的营养部门的产品开发而准备好使用实践/技术来采用狮子蘑菇种植。