Mechanism and structural dynamics of translation

拟议的研究重点是负责将遗传信息翻译成功能蛋白质的细胞机制的结构和功能动力学，这一过程称为翻译。翻译发生在核糖体，一个大的大分子复合物，它在一个复杂的和精确定时的方式与几个额外的蛋白质因子相互作用，以实现快速和有效的蛋白质合成在活细胞中观察到。为了研究核糖体上翻译因子的功能和结构动力学;将采用多学科方法研究纯化的体外翻译系统，该方法将经典生物化学和荧光光谱学等技术与分子动力学计算等计算技术相结合。

The proposed research focuses on the structural and functional dynamics of the cellular machinery that is responsible for the translation of genetic information into functional proteins, a process called translation. Translation takes place at the ribosome, a large macromolecular complex, which in a complex and precisely timed fashion interacts with several additional protein factors to achieve the fast and efficient protein synthesis observed in the living cell. To study the functional and structural dynamics of translation factors on the ribosomes; a purified in vitro translation system will be studied applying a multidisciplinary approach combining techniques ranging form classical biochemistry and fluorescence spectroscopy, to computational techniques such as molecular dynamics calculations.