Time-resolved, multi-angle light scattering instrumentation for characterization of nanoparticles and biomolecular assemblies

该提案的目的是获得时间分辨，多角度光散射（MALS）仪器，用于表征uOttawa生物，化学和物理系的纳米粒子和生物分子组装。 MALS是实时测定溶液中大分子、胶束、生物分子组装体和纳米颗粒的尺寸、形状、绝对摩尔质量和两体相互作用参数的必要实验技术。 此外，该系统的时间分辨能力将允许研究生物分子、脂质和/或纳米颗粒组装成超分子复合物的动力学。 这种仪器是生物学，生物物理学，分子生物化学，生物材料和定量生物医学中越来越多的跨学科研究计划所必需的。Wyatt Technology的MALS是一个独特的多功能和强大的系统，可用于获得溶液中各种生物大分子，生物分子组装体和纳米颗粒的详细，实时散射数据。除了对本提案中描述的具体研究计划的重要影响外，收购该MALS系统将加强科学学院，环境基因组学高级研究中心和uOttawa的NSERC CREATE培训计划中的跨学科研究和培训工作。

The aim of this proposal is to acquire time-resolved, multi-angle light scattering (MALS) instrumentation for the characterization of nanoparticles and biomolecular assemblies in the Departments of Biology, Chemistry, and Physics at uOttawa. MALS is an essential experimental technique for real-time determination of size, shape, absolute molar mass, and two-body interaction parameters of macromolecules, micelles, biomolecular assemblies, and nanoparticles in solution. Moreover, the time-resolved capabilities of this system will allow for the study of the kinetics of assembly of biomolecules, lipids, and/or nanoparticles into supramolecular complexes. This instrumentation is required for a growing number of interdisciplinary research programs in biology, biophysics, molecular biochemistry, biomaterials, and quantitative biomedicine. The requested MALS from Wyatt Technology is a uniquely versatile and powerful system that can be used to obtain detailed, real-time scattering data for wide variety of biomacromolecules, biomolecular assemblies and nanoparticles in solution. Beyond its important impact on the specific research programs described in this proposal, acquisition of this MALS system will enhance interdisciplinary research and training efforts within the Faculty of Science, the Centre for Advanced Research in Environmental Genomics, and the NSERC CREATE training programs in "Quantitative Biomedicine" and "Medicinal Chemistry and Biopharmaceutical Development" at uOttawa.