Role of chromatin in cellular differentiation.

成熟组织或特定细胞的形成是最复杂的生物过程之一，需要一个协调的基因表达程序。成熟组织形成的起始不仅受组织特异性转录调控蛋白的控制，还受多个不同水平的重要细胞修饰的控制，尤其是在染色质结构水平上。我在这项提案中概述的研究旨在更好地了解染色质如何调节对细胞分化至关重要的基因。这项提议将首先关注脂肪生成，这是一种由核受体PPAR-伽马控制的分化系统。我们将使用不同的分子和细胞方法来表征染色质结构在脂肪形成过程中受PPAR-伽马调控的基因调控中的作用。这些研究将为导致多细胞生物体形成的染色质相关分子机制提供新的信息。

The formation of mature tissues or specific cells represents one of the most complicated biological processes that requires a coordinated gene expression program. The initiation of the formation of a mature tissues is controlled by not only tissue-specific transcriptional regulatory proteins, but also by important cellular modifications at several different levels, more particularly at the level of chromatin structure. The studies that I outline in this proposal are aimed at acquiring an improved understanding of how chromatin regulates genes important for cellular differentiation. This proposal will first focus on adipogenesis, a differentiation system controlled by the nuclear receptor PPAR-gamma. We will use different molecular and cellular approaches to characterise the role of chromatin structure in the regulation of gene regulated by PPAR-gamma during adipogenesis. These studies will provide novel information on the as yet poorly characterised chromatin-related molecular mechanisms leading to the formation of a multi-cellular organism.