Flavonol deglycosylation in arabidopsis

测序基因组和代谢物图谱技术的可用性为科学家提供了研究植物次生代谢所需的工具。在植物中，类黄酮的积累受发育和环境胁迫因素的调节。在许多植物中，黄酮醇糖苷结合物作为类黄酮生物合成的主要最终产物和储存池而积累。有趣的是，在植物生理决定的过程中，去结合的黄酮醇苷元池增加。尽管有许多研究描述了促进黄酮醇生物合成的基因和酶，但关于控制黄酮醇脱糖基化和周转的生化过程的信息很少。在过去的几十年里，越来越多的证据表明，糖基水解酶介导了生物活性的苷元从糖苷结合的黄酮类化合物中释放出来。拟南芥已经成为类黄酮生物化学和遗传学的典范。我们将分析和描述在植物发育和环境胁迫适应过程中黄酮苷转化为苷元库的机制。

The availability of sequenced genomes and metabolite profiling technologies offer scientists the required tools to investigate secondary metabolism in plants. In plants, flavonoid accumulation is regulated by developmental and environmental stress factors. In many plants, flavonol glycoside conjugates accumulate as major end products and storage pools of flavonoid biosynthesis. Interestingly, the deconjugated flavonol aglycone pool is increased during physiologically determined processes in plants. Although, there are numerous studies describing the genes and enzymes promoting flavonol biosynthesis, very little information exists on the biochemical processes that govern flavonol deglycosylation and turnover. Over the past few decades, there is mounting evidence that glycosyl hydrolases mediate the liberation of bioactive aglycones from glycoside conjugated flavonoids. Arabidopsis thaliana has become a model for flavonoid biochemistry and genetics. We will analyze and describe the mechanisms involved in the conversion of flavonol glycoside to aglycone pools during plant development and environmental stress acclimation.