Bio-manufacturing of tissue engineering scaffolds

组织工程的一个关键目标是生产可以与患者一起生长的“人造”器官和组织替代品，最终为受损的器官或组织提供永久的解决方案。为此，组织支架发挥了重要作用。组织支架是由具有高度互连的孔隙网络的生物材料制成的三维（3D）结构，并且用于促进细胞生长和营养物和废物的运输。由于难以模拟天然组织的组织结构，创建这些支架已被证明具有挑战性。在这个项目中，我们将开发一种生物制造组织支架的方法，其中活细胞被操纵并添加到支架中。用快速成型方法来描述这种方法，在这种方法中，3D支架在连续的薄层中制造，我们将能够制造具有空间控制的细胞和支架微结构分布的支架。

One key goal of tissue engineering is to produce 'artificial' organ and tissue substitutes that can grow with patients, ultimately providing a permanent solution to damaged organs or tissues. For this, tissue scaffolds play an important role. A tissue scaffold is a three-dimensional (3D) structure made from biomaterials with highly interconnected pore networks, and is used to facilitate cell growth and transport of nutrients and wastes. Creating these scaffolds has proven challenging due to the difficulty in mimicking the organization of native tissues. In this project, we will develop a method for bio-manufacturing tissue scaffolds, in which living cells are manipulated and added to the scaffolds. Incorporating the method with rapid prototyping, an approach in which a 3D scaffold is manufactured in successive thin layers, we will be able to fabricate scaffolds with spatially controlled distributions of cells and scaffold microstructure.