Real Time Polymerase Chain Reaction Machine

本RTI申请的主要申请人是大卫赖特博士，大卫戴克博士、杰里米辛普森博士和阿曼达赖特博士是共同申请人。D.Wright博士的实验室研究了运动和饮食对脂肪组织和肝功能和代谢的调节作用。Wright博士的基础研究计划的主要目标是通过NSERC发现资助，阐明胰高血糖素在控制肝源性激素（称为肝细胞因子）分泌中的作用和潜在机制，并反过来研究这些改变如何影响全身碳水化合物和脂质代谢。肝细胞因子基因表达的分析是这些实验的中心结果测量。Wright博士的研究与Dyck博士的研究实验室正在进行的NSERC资助的研究相吻合，该研究重点是控制骨骼肌中碳水化合物和脂质的摄取，氧化和储存的基本机制，以及这些复杂的过程如何受到脂肪组织衍生的信号因子（称为脂肪因子）的调节。Dyck博士和D.Wright博士的机械代谢研究项目补充了Jeremy Simpson博士的NSERC资助项目，该项目研究了骨骼肌收缩蛋白的翻译后修饰以及这些蛋白如何通过衰老，饮食和运动改变。最后，阿曼达赖特博士的NSERC资助的研究旨在更好地了解脂质分子结构和物理性质对调节营养功能的吸收前消化事件的基本作用。总的来说，本提案申请人的研究计划需要广泛分析基因表达的变化，因为它涉及多个系统，包括骨骼肌、脂肪组织、肝脏和肠道功能以及代谢。因此，申请人要求提供资金，以购买一台新的RT-PCR机器进行这些测量。申请人实验室正在进行的研究产生的数据将增加我们对在各种营养和生理应激下控制骨骼肌、脂肪组织、肝脏和肠道功能和代谢的机制的基本理解。

The principal applicant for this RTI application is Dr. David Wright with Drs. David Dyck, Jeremy Simpson and Amanda Wright serving as co-applicants. Dr. D.Wright's laboratory examines the mediators of exercise and diet on the regulation of adipose tissue and liver function and metabolism. The main objective of Dr. Wright's basic research program, which is funded through an NSERC Discovery Grant, is to elucidate the role and underlying mechanisms of the hormone glucagon in controlling the secretion of liver derived hormones called hepatokines, and, in turn, examining how these alterations impact whole body carbohydrate and lipid metabolism. The analysis of hepatokine gene expression is a central outcome measure of these experiments. Dr. D.Wright's research dovetails with the on-going NSERC-funded investigations in Dr. Dyck's research laboratory which focuses on the basic mechanisms controlling the uptake, oxidation and storage of carbohydrate and lipid in skeletal muscle and how these intricate processes are regulated by adipose tissue derived signaling factors called adipokines. The mechanistic, metabolic research programs of Drs Dyck and D.Wright complement Dr. Jeremy Simpson's NSERC funded program that examines the post-translational modifications of contractile proteins in skeletal muscle and how these are altered by aging, diet and exercise. Finally Dr. Amanda Wright's NSERC funded research seeks to better understand the fundamental role of lipid molecular structure and physical properties on pre-absorptive digestive events that regulate nutritional functionality. Collectively, the research programs of the applicants of this proposal require the extensive analysis of changes in gene expression as it relates to multiple systems, including skeletal muscle, adipose tissue, liver and gut function and metabolism. Thus, the applicants are requesting funding to purchase a new RT-PCR machine for these measurements. The data generated from the ongoing studies in the applicants laboratories will increase our basic understanding of the mechanisms controlling skeletal muscle, adipose tissue, liver and gut function and metabolism under a variety of nutritional and physiological stresses.