"Sensing, transfer and regulation of ions and gases: Studies in a model organism, the zebrafish (Danio rerio)"

这项研究计划侧重于鱼类适应波动环境的两个至关重要的关键方面;调节体液的离子组成以及呼吸气体O2和CO2的传感和传输。虽然我们已经了解了很多关于离子在上皮细胞（如鳃和皮肤）中运动的机制，但实际上对离子调节的代谢成本一无所知--离子在细胞中运动的成本（能量）是多少？ 我将研究这个问题，使用离子吸收的表皮离子转运细胞的斑马鱼幼虫作为一个模型系统。我和我的学员将使用最先进的扫描极谱和罐装离子选择性电极技术，同时测量离子和O2流入离子细胞，以提供离子调节代谢成本的第一个直接估计。 离子调节领域的其他研究将集中在建立调节机制，使鱼类能够在环境挑战期间调整离子吸收率，例如降低环境O2（缺氧）和改变盐度。

This research proposal focuses on two key aspects of fundamental importance to the adaptation of fish to fluctuating environments; regulating the ionic composition of their body fluids and the sensing and transfer of the respiratory gases O2 and CO2. While we have learned much about the mechanisms of ion movement across epithelial such as gills and skin, virtually nothing is know about the metabolic cost of ion regulation - what does it cost (in energy) to move ions across a cell? I will examine this question using ionic uptake by the epidermal ion-transporting cells of zebrafish larvae as a model system. My trainees and I will use state-of-the-art scanning polarographic- and canning ion-selective electrode techniques to simultaneously measure ion and O2 fluxes into ionocytes to provide the first direct estimates of the metabolic cost of ion regulation. Additional research in the field of ion regulation will concentrate on establishing the regulatory mechanisms allowing fish to adjust ion uptake rates during environmental challenges such as lowered ambient O2 (hypoxia) and changing salinity.