DNA-Protein binding assays

DNA和蛋白质之间的相互作用负责细胞中的许多生物过程。这些相互作用的亲和力和特异性可以用于利用dna -蛋白质结合的生物分析分析。本研究的主要目的是开发结合dna -蛋白质亲和识别、毛细管电泳分离和激光诱导荧光检测的新型生物分析技术。已建立的和新的亲和探针，包括抗体、DNA损伤识别蛋白和短合成DNA分子（适体），将检查它们与目标分子结合的能力。例如，为了检测DNA损伤，将DNA从细胞中分离出来，并与识别损伤的荧光探针孵育。通过毛细管电泳将亲和探针与受损DNA的复合物与其他分子分离，并通过激光诱导荧光测定其浓度。

Interactions between DNA and proteins are responsible for many biological processes in the cell. The affinity and specificity of these interactions can be exploited for bioanalytical assays that make use of DNA-protein binding. The primary objective of this research is to develop novel bioanalytical technology combining DNA-protein affinity recognition, capillary electrophoresis separation, and laser induced fluorescence detection. Both established and novel affinity probes, including antibodies, DNA damage recognition proteins, and short synthetic DNA molecules (aptamers), will be examined for their ability to bind with the target molecules. To assay for DNA damage, for example, DNA will be isolated from cells and incubated with a fluorescent probe recognizing the damage. The complex of the affinity probe with the damaged DNA will be separated from other molecules by capillary electrophoresis and its concentration will be quantified by laser induced fluorescence.