Molecular architecture in gene expression and virus-host interactions

我研究的目的是了解蛋白质如何组装成一个非常适合特定功能的结构。可视化蛋白质组装差异所需的原子级细节是通过使用我实验室当地可用的NMR光谱学和X射线晶体学等技术实现的。我的建议中的三个目标涵盖了细胞信号传导的三个一般领域。第一个目的是研究一对Sox 9蛋白如何共同作用，能够激活细胞中不同的发育程序，而不是一个Sox 9蛋白单独工作。第二个目的是研究细菌转录因子及其驱动抗生素耐药性的启动子之间相互作用的性质。第三个目标是研究在病毒感染过程中，一种叫做ea8.5的蛋白质是如何改变细菌的遗传程序的。该提案中描述的基础研究将用于了解细胞信号传导的新方面，并帮助设计针对疾病的选择性疗法。

The aim of my research is to understand how proteins assemble into an architecture that is well suited to a specific function. The atomic level of detail required to visualize differences in protein assemblies is achieved by using techniques such as NMR spectroscopy and X-ray crystallography that are available locally to my laboratory. The three aims in my proposal cover three general areas of cell signaling. The first aim examines how a pair of Sox9 proteins working together is capable of activating a different developmental program in a cell than one Sox9 protein working alone. The second aim examines the nature of the interactions between a bacterial transcription factor and its promoters that drive antibiotic resistance. The third aim examines how a bacterial genetic program is altered during viral infection by a protein called ea8.5. The basic research described in this proposal will be used to understand the new aspects of cell signaling and help design selective therapies against disease.