Aptamer-facilitated technologies for cell activity modulation and imaging

生物分析化学和生物技术最具挑战性的任务之一是用人工竞争对手取代动物或杂交瘤制造的抗体，这些竞争对手可以很容易地选择和化学合成。这类竞争对手是适配子--单链DNA或RNA寡核苷酸，可以与各种不同的靶标结合，从小分子到具有高亲和力和特异性的蛋白质。适配子是一种具有改变亲和探针领域和取代抗体作为诊断和治疗试剂的巨大潜力的配体。与抗体不同的是，它们不会引发免疫反应。它们具有热稳定性，可以以较低的价格进行化学合成，具有更好的批次到批次的重现性，并且具有化学修饰和集成到生物传感器中的简单性。

One of the most challenging tasks of bioanalytical chemistry and biotechnology is the replacement of animal- or hybridoma-made antibodies with artificial competitors that can be easily selected and chemically synthesized. Such competitors are aptamers - single-stranded DNA or RNA oligonucleotides that can bind to a variety of different targets ranging from small molecules to proteins with high affinity and specificity. Aptamers are ligands with big potential to change the field of affinity probes and to replace antibodies as diagnostic and therapeutic reagents. In contrast to antibodies, they do not trigger an immune response. They are thermostable and can be chemically synthesized for lower price, have better batch-to-batch reproducibility, and have the simplicity of chemical modifications and integration in biosensors.