Advanced Neurostereology System for Integrative Neurobiology Group

这是一份修订后的申请，旨在获得资金升级现有的显微镜设备，以适应西安大略大学一组综合神经生物学家的先进神经立体学系统。一组nserc资助的实验室试图用一个新的彩色成像相机和MicroBrightField的立体探测器系统来升级共享的尼康Eclipse 90i显微镜。这种最先进的神经立体系统将提供一种经济有效的解决方案，以获得准确、无偏的数量、长度、面积和大脑组织切片中细胞或生物结构的体积。MicroBrightField的立体调查员系统是世界上最先进的神经立体系统。这个系统，也没有任何类似的系统，是可用的西方或任何其他机构在伦敦，安大略地区。该请求中包含了一个扩展模块，以便每个使用通用数据采集系统的研究实验室可以在远离显微镜的情况下进行立体分析。这将大大提高普通显微镜的效率，因为显微镜用于数据采集不会妨碍其他研究人员进行数据分析。总的来说，这个数据采集和分析系统的完成将是我们联合研究计划的一个重大进步，并将使我们所有的nserc资助的项目受益。西部大学的综合神经生物学小组包括多个系（心理学、生理学和药理学、解剖学和细胞生物学、听力学）和多个院系（社会科学、医学和牙科、健康科学）的实验室。这些设备将由HQP在六个不同实验室的各级使用，以及来自其他实验室的学员在需要时能够使用这些设备。总的来说，购置这种设备将对可推进的研究和对许多研究学科现有培训机会的广度产生直接和持久的影响。

This is a revised application to obtain funds to upgrade existing microscopy equipment to accommodate an advanced neurostereology system for a group of integrative neurobiologists at the University of Western Ontario. A group of NSERC-funded labs seeks to upgrade a shared Nikon Eclipse 90i microscope with a new colour imaging camera and a Stereo Investigator system from MicroBrightField. This state-of-the-art advanced neurostereology system will provide a cost-effective solution to acquire accurate, unbiased estimates of the number, length, area, and volume of cells or biological structures in a brain tissue section. The Stereo Investigator system from MicroBrightField is the world's foremost system for advanced neurostereology. Neither this system, nor any similar system, is available at Western or from any other institution in the London, Ontario area. Included in this request is an extension module so that each research lab using the common data acquisition system can perform their stereological analysis away from the microscope. This will greatly improve the efficiency of the common microscope, as microscope use for data acquisition will not prevent other researchers from performing data analysis. In total, completion of this data acquisition and analysis system will be a significant advance for our combined research programs and will benefit all of our NSERC-sponsored projects. The Integrative Neurobiology Group at Western includes laboratories across multiple departments (Psychology, Physiology and Pharmacology, Anatomy & Cell Biology, and Audiology) and multiple faculties (Social Science, Medicine & Dentistry, and Health Science). This equipment will be utilized by HQP at all levels, across six different laboratories, as well as trainees from other laboratories that will be able to access the equipment as needed. Overall, the acquisition of this equipment will have both an immediate and long-lasting effect on the research that can be advanced and on the breadth of the training opportunities available in many research disciplines.